Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 5.4.3.8 extracted from

  • Smith, M.A.; Grimm, B.
    Gabaculine resistance of Synechococcus glutamate 1-semialdehyde aminotransferase (1992), Biochemistry, 31, 4122-4127.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
wild-type enzyme and gabaculine-resistant mutant enzyme, overexpression in Escherichia coli Synechococcus sp.

Protein Variants

Protein Variants Comment Organism
additional information gabaculine-resistant mutant enzyme with a 3 times lower catalytic efficiency and impaired prototropic rearrangement and transaldimination Synechococcus sp.

Inhibitors

Inhibitors Comment Organism Structure
gabaculine wild-type enzyme is inhibited, gabaculine-resistant mutant enzyme is not inhibited Synechococcus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.022
-
(S)-4-Amino-5-oxopentanoate gabaculine-resistant mutant enzyme Synechococcus sp.
0.046
-
(S)-4-Amino-5-oxopentanoate wild-type enzyme Synechococcus sp.

Organism

Organism UniProt Comment Textmining
Synechococcus sp.
-
wild-type strain and gabaculine-resistant strain
-

Purification (Commentary)

Purification (Comment) Organism
wild-type enzyme and gabaculine-resistant mutant enzyme Synechococcus sp.

Reaction

Reaction Comment Organism Reaction ID
L-glutamate 1-semialdehyde = 5-aminolevulinate wild-type enzyme and gabaculine-resistant mutant enzyme use a ping-pong bi-bi mechanism in which 4,5-diaminovalerate is a likely intermediate Synechococcus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-4-Amino-5-oxopentanoate
-
Synechococcus sp. 5-Amino-4-oxopentanoate
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.077
-
(S)-4-Amino-5-oxopentanoate gabaculine-resistant mutant enzyme Synechococcus sp.
0.47
-
(S)-4-Amino-5-oxopentanoate wild-type enzyme Synechococcus sp.

Cofactor

Cofactor Comment Organism Structure
vitamin B6 wild-type enzyme and gabaculine-resistant mutant enzyme contain vitamin B6 Synechococcus sp.