BRENDA - Enzyme Database
show all sequences of 5.4.3.8

Crystal structure of a glutamate-1-semialdehyde-aminomutase from Pseudomonas aeruginosa PAO1

Li, S.; Lou, X.; Xu, Y.; Teng, X.; Che, S.; Liu, R.; Bartlam, M.; Biochem. Biophys. Res. Commun. 500, 804-809 (2018)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
gene pa4088, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
Pseudomonas aeruginosa
Crystallization (Commentary)
Crystallization (Commentary)
Organism
purified recombinant His-tagged wild-type enzyme and enzyme mutant K286A in complex with pyridoxamine 5'-phosphate cofactor, mixing of 10 mg/ml protein in 20 mM Tris, 200 mM NaCl, pH 8.0, with an equal volume of reservoir solution consisting of 0.2M ammonium sulfate, 0.1 M Bis-Tris, pH 6.5, and 25% PEG 3350 for the wild-type enzyme, and of 0.1 M Tris, pH 8.5, and 25% PEG3350 for the mutant enzyme, 20C, 1 week, X-ray diffraction structure determination and analysis at 1.44-1.57 A resolution
Pseudomonas aeruginosa
Engineering
Protein Variants
Commentary
Organism
K286A
site-directed mutagenesis
Pseudomonas aeruginosa
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
L-glutamate 1-semialdehyde
Pseudomonas aeruginosa
-
5-aminolevulinate
-
-
?
L-glutamate 1-semialdehyde
Pseudomonas aeruginosa ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1
-
5-aminolevulinate
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Pseudomonas aeruginosa
Q9HWU0
-
-
Pseudomonas aeruginosa ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1
Q9HWU0
-
-
Purification (Commentary)
Purification (Commentary)
Organism
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration
Pseudomonas aeruginosa
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
L-glutamate 1-semialdehyde
-
747005
Pseudomonas aeruginosa
5-aminolevulinate
-
-
-
?
L-glutamate 1-semialdehyde
-
747005
Pseudomonas aeruginosa ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1
5-aminolevulinate
-
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
three-dimensional structure analysis of wild-type and mutant enzymes, overview
Pseudomonas aeruginosa
Synonyms
Synonyms
Commentary
Organism
glutamate-1-semialdehyde-aminomutase
-
Pseudomonas aeruginosa
PA4088
-
Pseudomonas aeruginosa
protein PA4088
-
Pseudomonas aeruginosa
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
in both the pyridoxamine 5'-phosphate- and pyridoxal 5'-phosphate-bound structures, the gating loops are well-defined in electron density and are in the ordered open conformation. The conformation of the gating loop is symmetrical
Pseudomonas aeruginosa
pyridoxal 5'-phosphate
a pyridoxamine 5'-phosphate (PMP)/pyridoxal 5'-phosphate (PLP)-dependent enzyme, binding structure with a Schiff base linkage between the cofactor and the epsilon-amino group of Lys286, overview
Pseudomonas aeruginosa
pyridoxamine 5'-phosphate
a pyridoxamine 5'-phosphate (PMP)/pyridoxal 5'-phosphate (PLP)-dependent enzyme, binding structure, overview
Pseudomonas aeruginosa
Cloned(Commentary) (protein specific)
Commentary
Organism
gene pa4088, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
Pseudomonas aeruginosa
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
in both the pyridoxamine 5'-phosphate- and pyridoxal 5'-phosphate-bound structures, the gating loops are well-defined in electron density and are in the ordered open conformation. The conformation of the gating loop is symmetrical
Pseudomonas aeruginosa
pyridoxal 5'-phosphate
a pyridoxamine 5'-phosphate (PMP)/pyridoxal 5'-phosphate (PLP)-dependent enzyme, binding structure with a Schiff base linkage between the cofactor and the epsilon-amino group of Lys286, overview
Pseudomonas aeruginosa
pyridoxamine 5'-phosphate
a pyridoxamine 5'-phosphate (PMP)/pyridoxal 5'-phosphate (PLP)-dependent enzyme, binding structure, overview
Pseudomonas aeruginosa
Crystallization (Commentary) (protein specific)
Crystallization
Organism
purified recombinant His-tagged wild-type enzyme and enzyme mutant K286A in complex with pyridoxamine 5'-phosphate cofactor, mixing of 10 mg/ml protein in 20 mM Tris, 200 mM NaCl, pH 8.0, with an equal volume of reservoir solution consisting of 0.2M ammonium sulfate, 0.1 M Bis-Tris, pH 6.5, and 25% PEG 3350 for the wild-type enzyme, and of 0.1 M Tris, pH 8.5, and 25% PEG3350 for the mutant enzyme, 20C, 1 week, X-ray diffraction structure determination and analysis at 1.44-1.57 A resolution
Pseudomonas aeruginosa
Engineering (protein specific)
Protein Variants
Commentary
Organism
K286A
site-directed mutagenesis
Pseudomonas aeruginosa
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
L-glutamate 1-semialdehyde
Pseudomonas aeruginosa
-
5-aminolevulinate
-
-
?
L-glutamate 1-semialdehyde
Pseudomonas aeruginosa ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1
-
5-aminolevulinate
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration
Pseudomonas aeruginosa
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
L-glutamate 1-semialdehyde
-
747005
Pseudomonas aeruginosa
5-aminolevulinate
-
-
-
?
L-glutamate 1-semialdehyde
-
747005
Pseudomonas aeruginosa ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1
5-aminolevulinate
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
three-dimensional structure analysis of wild-type and mutant enzymes, overview
Pseudomonas aeruginosa
General Information
General Information
Commentary
Organism
evolution
the enzyme is a member of the GSAM family
Pseudomonas aeruginosa
additional information
three-dimensional structure analysis of wild-type and mutant enzymes, overview. In both the pyridoxamine 5'-phosphate- and pyridoxal 5'-phosphate-bound structures, the gating loops are well-defined in electron density and are in the ordered open conformation. The conformation of the gating loop is symmetrical
Pseudomonas aeruginosa
General Information (protein specific)
General Information
Commentary
Organism
evolution
the enzyme is a member of the GSAM family
Pseudomonas aeruginosa
additional information
three-dimensional structure analysis of wild-type and mutant enzymes, overview. In both the pyridoxamine 5'-phosphate- and pyridoxal 5'-phosphate-bound structures, the gating loops are well-defined in electron density and are in the ordered open conformation. The conformation of the gating loop is symmetrical
Pseudomonas aeruginosa
Other publictions for EC 5.4.3.8
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
747005
Li
Crystal structure of a glutam ...
Pseudomonas aeruginosa, Pseudomonas aeruginosa ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1
Biochem. Biophys. Res. Commun.
500
804-809
2018
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746660
Song
Crystal structure of glutamat ...
Arabidopsis thaliana
Acta Crystallogr. Sect. F
72
448-456
2016
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1
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747678
Ramzi
5-Aminolevulinic acid product ...
Corynebacterium glutamicum, Corynebacterium glutamicum ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025
Enzyme Microb. Technol.
81
1-7
2015
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727125
Campanini
Asymmetry of the active site l ...
Synechococcus sp.
BioMed Res. Int.
2013
353270
2013
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716103
Kang
Engineering Escherichia coli f ...
Escherichia coli, Escherichia coli MG1655
Metab. Eng.
13
492-498
2011
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28
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703619
Orriss
Absence of a catalytic water c ...
Synechococcus sp.
FASEB J.
24
404-414
2010
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714041
Ge
Crystal structure of glutamate ...
Bacillus subtilis
Biochem. Biophys. Res. Commun.
402
356-360
2010
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1
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677347
Lv
Cloning, expression, purificat ...
Bacillus subtilis
Acta Crystallogr. Sect. F
62
483-485
2006
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682509
Stetefeld
Intersubunit signaling in glut ...
Synechococcus sp.
Proc. Natl. Acad. Sci. USA
103
13688-13693
2006
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1
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662360
Ler
Complex formation between glut ...
Escherichia coli
J. Biol. Chem.
280
18568-18572
2005
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653764
Tsang
Expression of a Brassic napus ...
Brassica napus
Protein Expr. Purif.
29
193-201
2003
1
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649844
Smith
Transient-state kinetic analys ...
Synechococcus sp.
Biochemistry
37
319-329
1998
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3445
Grimm
-
Crystal structure of glutamate ...
Synechococcus sp.
Proc. Natl. Acad. Sci. USA
84
4866-4871
1997
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3440
Palmieri
Glutamate-1-semialdehyde amino ...
Saccharolobus solfataricus, Saccharolobus solfataricus P2
Biochem. J.
320
541-545
1996
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3435
Brody
Characterization of the differ ...
Synechococcus sp.
Biochemistry
34
15918-15924
1995
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3421
Henning
Crystallization and preliminar ...
Synechococcus sp.
J. Mol. Biol.
242
591-594
1994
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3434
Mayer
Metal requirement of the enzym ...
Chlorella vulgaris, Synechococcus sp.
Arch. Biochem. Biophys.
312
203-209
1994
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721440
Matters
-
Biosynthesis of delta-aminolev ...
Saccharolobus solfataricus, Saccharolobus solfataricus 98-3
Arch. Microbiol.
161
272-276
1994
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3442
Tyacke
Properties of the pyridoxaldim ...
Pisum sativum
Biochem. J.
293
697-791
1993
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2
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3443
Murakami
Cloning and characterization o ...
Propionibacterium freudenreichii
Appl. Environ. Microbiol.
59
347-350
1993
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1
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1
1
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3444
Murakami
Cloning and characterization o ...
Xanthomonas campestris
Appl. Microbiol. Biotechnol.
38
502-506
1993
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1
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1
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1
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1
1
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3427
Berry-Lowe
Purification and characterizat ...
Hordeum vulgare
Plant Physiol.
99
1597-1603
1992
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1
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1
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5
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1
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1
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3
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1
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1
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3
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1
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3430
Smith
Gabaculine resistance of Synec ...
Synechococcus sp.
Biochemistry
31
4122-4127
1992
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1
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1
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1
2
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3
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1
1
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1
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2
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1
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1
1
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1
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1
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1
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1
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2
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3432
Ilag
Activity and spectroscopic pro ...
Escherichia coli
Biochemistry
31
7143-7151
1992
2
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1
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1
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1
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2
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4
1
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2
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1
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1
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1
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4
1
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3433
Smith
Glutamate 1-semialdehyde amino ...
Synechococcus sp.
Biochemistry
31
11249-11254
1992
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1
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1
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3439
Pugh
Mechanism of glutamate semiald ...
Pisum sativum
J. Biol. Chem.
267
1584-1588
1992
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1
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1
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1
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1
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3
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3426
Rieble
Separation and partial charact ...
Synechococcus sp.
Arch. Biochem. Biophys.
289
289-297
1991
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1
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1
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2
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1
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1
1
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1
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2
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3436
Jahn
Purification and functional ch ...
Chlamydomonas reinhardtii
J. Biol. Chem.
266
161-167
1991
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2
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1
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1
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2
1
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1
1
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1
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1
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2
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1
1
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1
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1
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2
1
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1
1
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3438
Nair
Direct identification and quan ...
Pisum sativum
FEBS Lett.
283
4-6
1991
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2
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1
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1
1
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2
1
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1
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1
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2
1
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1
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1
1
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2
1
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3441
Smith
Characterization of glutamate- ...
Synechococcus sp.
Eur. J. Biochem.
202
749-757
1991
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1
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3
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1
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1
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1
1
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1
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3
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3
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1
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3429
Bull
-
Cyanobacterial glutamate 1-sem ...
Synechococcus sp.
Arch. Microbiol.
154
56-59
1990
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3
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1
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3431
Nair
Inhibition studies on 5-amino- ...
Pisum sativum
Biochem. Soc. Trans.
18
656-657
1990
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3437
Grimm
Primary structure of a key enz ...
Hordeum vulgare
Proc. Natl. Acad. Sci. USA
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4169-4173
1990
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1
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2
1
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2
1
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3428
Grimm
Purification and partial amino ...
Hordeum vulgare, Synechococcus sp.
Carlsberg Res. Commun.
54
67-79
1989
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6
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2
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2
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3424
Mau
Biosynthesis of delta-aminolev ...
Chlamydomonas reinhardtii
Plant Physiol.
86
793-797
1988
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1
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3425
Hoober
Biosynthesis of DELTA-aminolev ...
Hordeum vulgare
Carlsberg Res. Commun.
53
11-25
1988
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1
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1
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1
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1
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1
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1
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1
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1
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3423
Kannangara
-
Biosynthesis of DELTA-aminolev ...
Hordeum vulgare
Carlsberg Res. Commun.
50
179-191
1985
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1
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1
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2
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1
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1
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1
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2
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3422
Wang
Purification, characterization ...
Chlamydomonas reinhardtii
Plant Physiol.
74
569-575
1984
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1
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1
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1
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1
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3420
Kannangara
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Biosynthesis of DELTA-aminolev ...
Hordeum vulgare
Carlsberg Res. Commun.
44
11-20
1979
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1
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1
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1
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2
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1
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1
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1
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2
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3419
Kannangara
-
Biosynthesis of DELTA-aminolev ...
Hordeum vulgare
Carlsberg Res. Commun.
43
185-194
1978
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7
1
2
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2
1
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1
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1
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1
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2
1
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1
1
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1
1
-
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-
-
-
-
-
7
-
1
2
-
2
1
-
-
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1
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1
-
-
2
1
1
1
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1
1
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-
-
-
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-