BRENDA - Enzyme Database show
show all sequences of 5.4.3.6

Kinetic analysis of the 4-methylideneimidazole-5-one-containing tyrosine aminomutase in enediyne antitumor antibiotic C-1027 biosynthesis

Christenson, S.D.; Wu, W.; Spies, M.A.; Shen, B.; Toney, M.D.; Biochemistry 42, 12708-12718 (2003)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli
Streptomyces globisporus
Engineering
Amino acid exchange
Commentary
Organism
S153A
1.5% of wild-type activity
Streptomyces globisporus
S153C
5% of wild-type activity
Streptomyces globisporus
Inhibitors
Inhibitors
Commentary
Organism
Structure
Cd2+
weak inhibition
Streptomyces globisporus
Cu2+
strong inhibition
Streptomyces globisporus
Zn2+
weak inhibition
Streptomyces globisporus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0023
-
L-tyrosine
lyase activity
Streptomyces globisporus
0.028
-
L-tyrosine
-
Streptomyces globisporus
1.54
-
L-3,4-dihydroxyphenylalanine
-
Streptomyces globisporus
6.1
-
L-3-chlorotyrosine
-
Streptomyces globisporus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
58100
-
x * 58100, SDS-PAGE
Streptomyces globisporus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-tyrosine
Streptomyces globisporus
involved in the biosynthesis of the enediyne antitumor antibiotic C-1027
3-amino-3-(4-hydroxyphenyl)propanoate
-
Streptomyces globisporus
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Streptomyces globisporus
-
-
-
Purification (Commentary)
Commentary
Organism
recombinant tyrosine aminomutase
Streptomyces globisporus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(3S)-amino-3-(4-hydroxyphenyl)propanoate
beta-tyrosine racemase activity
650190
Streptomyces globisporus
(3R)-amino-3-(4-hydroxyphenyl)propanoate
-
650190
Streptomyces globisporus
r
L-3,4-dihydroxyphenylalanine
-
650190
Streptomyces globisporus
3-amino-3-(3,4-dihydroxyphenyl)propanoate
-
650190
Streptomyces globisporus
?
L-3-chlorotyrosine
-
650190
Streptomyces globisporus
3-amino-3-(3-chloro-4-hydroxyphenyl)propanoate
-
650190
Streptomyces globisporus
?
L-tyrosine
-
650190
Streptomyces globisporus
3-amino-3-(4-hydroxyphenyl)propanoate
-
650190
Streptomyces globisporus
r
L-tyrosine
involved in the biosynthesis of the enediyne antitumor antibiotic C-1027
650190
Streptomyces globisporus
3-amino-3-(4-hydroxyphenyl)propanoate
-
650190
Streptomyces globisporus
?
L-tyrosine
ammonia lyase activity of tyrosine aminomutase
650190
Streptomyces globisporus
(4-hydroxyphenyl)prop-2-enoate + NH3
-
650190
Streptomyces globisporus
?
Subunits
Subunits
Commentary
Organism
?
x * 58100, SDS-PAGE
Streptomyces globisporus
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.0012
-
L-tyrosine
lyase activity
Streptomyces globisporus
0.0048
-
L-3-chlorotyrosine
-
Streptomyces globisporus
0.0067
-
L-3,4-dihydroxyphenylalanine
-
Streptomyces globisporus
0.01
-
L-tyrosine
-
Streptomyces globisporus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.8
-
-
Streptomyces globisporus
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6.5
10.5
approx. 50% of maximal activity at pH 7.5 and pH 10.0, respectively
Streptomyces globisporus
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Streptomyces globisporus
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
S153A
1.5% of wild-type activity
Streptomyces globisporus
S153C
5% of wild-type activity
Streptomyces globisporus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Cd2+
weak inhibition
Streptomyces globisporus
Cu2+
strong inhibition
Streptomyces globisporus
Zn2+
weak inhibition
Streptomyces globisporus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0023
-
L-tyrosine
lyase activity
Streptomyces globisporus
0.028
-
L-tyrosine
-
Streptomyces globisporus
1.54
-
L-3,4-dihydroxyphenylalanine
-
Streptomyces globisporus
6.1
-
L-3-chlorotyrosine
-
Streptomyces globisporus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
58100
-
x * 58100, SDS-PAGE
Streptomyces globisporus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-tyrosine
Streptomyces globisporus
involved in the biosynthesis of the enediyne antitumor antibiotic C-1027
3-amino-3-(4-hydroxyphenyl)propanoate
-
Streptomyces globisporus
?
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant tyrosine aminomutase
Streptomyces globisporus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(3S)-amino-3-(4-hydroxyphenyl)propanoate
beta-tyrosine racemase activity
650190
Streptomyces globisporus
(3R)-amino-3-(4-hydroxyphenyl)propanoate
-
650190
Streptomyces globisporus
r
L-3,4-dihydroxyphenylalanine
-
650190
Streptomyces globisporus
3-amino-3-(3,4-dihydroxyphenyl)propanoate
-
650190
Streptomyces globisporus
?
L-3-chlorotyrosine
-
650190
Streptomyces globisporus
3-amino-3-(3-chloro-4-hydroxyphenyl)propanoate
-
650190
Streptomyces globisporus
?
L-tyrosine
-
650190
Streptomyces globisporus
3-amino-3-(4-hydroxyphenyl)propanoate
-
650190
Streptomyces globisporus
r
L-tyrosine
involved in the biosynthesis of the enediyne antitumor antibiotic C-1027
650190
Streptomyces globisporus
3-amino-3-(4-hydroxyphenyl)propanoate
-
650190
Streptomyces globisporus
?
L-tyrosine
ammonia lyase activity of tyrosine aminomutase
650190
Streptomyces globisporus
(4-hydroxyphenyl)prop-2-enoate + NH3
-
650190
Streptomyces globisporus
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 58100, SDS-PAGE
Streptomyces globisporus
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.0012
-
L-tyrosine
lyase activity
Streptomyces globisporus
0.0048
-
L-3-chlorotyrosine
-
Streptomyces globisporus
0.0067
-
L-3,4-dihydroxyphenylalanine
-
Streptomyces globisporus
0.01
-
L-tyrosine
-
Streptomyces globisporus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.8
-
-
Streptomyces globisporus
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6.5
10.5
approx. 50% of maximal activity at pH 7.5 and pH 10.0, respectively
Streptomyces globisporus
Other publictions for EC 5.4.3.6
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
747148
Attanayake
Understanding which residues ...
Oryza sativa
Biochemistry
57
3503-3514
2018
-
-
1
-
15
-
-
-
-
-
-
1
-
2
-
-
-
1
-
-
-
-
7
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
15
-
-
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-
-
-
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1
-
-
-
-
-
-
-
-
7
-
-
-
-
-
-
-
-
-
-
3
3
-
-
-
747104
Walter
Mutation of aryl binding pock ...
Oryza sativa
Biochemistry
55
3497-3503
2016
-
-
1
-
3
-
-
7
-
-
-
1
-
4
-
-
1
1
-
-
-
-
4
-
1
-
-
7
1
-
-
1
-
-
-
-
-
1
1
-
3
-
-
-
-
7
-
-
-
1
-
-
-
1
-
-
-
-
4
-
1
-
-
7
1
-
-
-
-
1
1
-
6
6
748914
Yan
The tyrosine aminomutase TAM1 ...
Oryza sativa Japonica Group
Plant Cell
27
1265-1278
2015
-
-
1
-
1
-
1
-
-
-
-
1
-
6
-
-
-
-
-
5
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
1
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-
-
-
1
-
-
-
-
-
5
-
-
1
-
-
-
-
-
-
-
-
-
2
3
3
2
-
-
727685
Wanninayake
A bacterial tyrosine aminomuta ...
Chondromyces crocatus
J. Am. Chem. Soc.
135
11193-11204
2013
-
-
1
-
-
-
-
-
-
-
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-
-
2
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1
1
-
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3
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1
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1
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1
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-
-
1
-
-
-
-
3
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
714481
Cooke
Probing the active site of MIO ...
Streptomyces globisporus
Biopolymers
93
802-810
2010
-
-
1
1
4
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
4
-
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1
-
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-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
714722
Wu
Engineering of an enantioselec ...
Chondromyces crocatus, Cupriavidus crocatus, Streptomyces globisporus
Chem. Commun. (Camb. )
46
8157-8159
2010
-
-
-
-
6
-
-
3
-
-
-
-
-
3
-
-
-
-
-
-
-
-
6
-
-
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3
-
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6
-
-
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-
3
-
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-
-
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-
-
-
-
-
6
-
-
-
-
3
-
-
-
-
-
-
-
-
3
3
703203
Krug
Discovery of additional member ...
Chondromyces crocatus, Myxococcus fulvus Mx f65, Myxococcus sp. Mx-B0
ChemBioChem
10
741-750
2009
-
-
3
-
12
-
-
2
-
-
-
3
-
6
-
-
3
-
-
-
-
-
4
-
3
-
-
4
3
-
-
-
-
-
-
-
-
3
-
-
12
-
-
-
-
2
-
-
-
3
-
-
-
3
-
-
-
-
4
-
3
-
-
4
3
-
-
-
-
1
1
-
-
-
691277
Montavon
Design and characterization of ...
Streptomyces globisporus
Bioorg. Med. Chem. Lett.
18
3099-3102
2008
-
-
-
-
-
-
6
-
-
-
-
-
-
1
-
-
-
-
-
-
-
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1
-
-
-
-
-
-
-
-
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-
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6
-
-
-
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-
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-
6
6
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-
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-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
678295
Christianson
The structure of L-tyrosine 2, ...
Streptomyces globisporus
Biochemistry
46
7205-7214
2007
-
-
1
1
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
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-
1
-
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1
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1
1
1
-
-
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-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
650190
Christenson
Kinetic analysis of the 4-meth ...
Streptomyces globisporus
Biochemistry
42
12708-12718
2003
-
-
1
-
2
-
3
4
-
-
1
1
-
1
-
-
1
-
-
-
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6
1
-
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4
1
1
-
-
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1
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2
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3
-
4
-
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1
1
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1
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6
1
-
-
-
4
1
1
-
-
-
-
-
-
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-
3406
Parry
-
Biosynthesis of amino acids. I ...
Brevibacillus brevis
J. Am. Chem. Soc.
102
836-837
1980
-
-
-
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1
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1
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3405
Kurylo-Borowska
Biosynthesis of beta-tyrosine ...
Brevibacillus brevis
Biochim. Biophys. Acta
264
1-10
1972
-
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1
8
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1
1
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1
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1
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1
1
2
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1
1
2
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1
1
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1
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1
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1
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8
-
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1
1
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1
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1
1
2
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1
1
2
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1
1
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