Literature summary for 5.4.3.6 extracted from

Christenson, S.D.; Wu, W.; Spies, M.A.; Shen, B.; Toney, M.D.
Kinetic analysis of the 4-methylideneimidazole-5-one-containing tyrosine aminomutase in enediyne antitumor antibiotic C-1027 biosynthesis (2003), Biochemistry, 42, 12708-12718.

Search for more literature for 5.4.3.6

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Streptomyces globisporus

Protein Variants

Protein Variants	Comment	Organism
S153A	1.5% of wild-type activity	Streptomyces globisporus
S153C	5% of wild-type activity	Streptomyces globisporus

Inhibitors

Inhibitors	Comment	Organism	Structure
Cd2+	weak inhibition	Streptomyces globisporus
Cu2+	strong inhibition	Streptomyces globisporus
Zn2+	weak inhibition	Streptomyces globisporus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0023	-	L-tyrosine	lyase activity	Streptomyces globisporus
0.028	-	L-tyrosine	-	Streptomyces globisporus
1.54	-	L-3,4-dihydroxyphenylalanine	-	Streptomyces globisporus
6.1	-	L-3-chlorotyrosine	-	Streptomyces globisporus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
58100	-	x * 58100, SDS-PAGE	Streptomyces globisporus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
L-tyrosine	Streptomyces globisporus	involved in the biosynthesis of the enediyne antitumor antibiotic C-1027	3-amino-3-(4-hydroxyphenyl)propanoate	-	?

Organism

Organism	UniProt	Comment	Textmining
Streptomyces globisporus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant tyrosine aminomutase	Streptomyces globisporus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(3S)-amino-3-(4-hydroxyphenyl)propanoate	beta-tyrosine racemase activity	Streptomyces globisporus	(3R)-amino-3-(4-hydroxyphenyl)propanoate	-	r
L-3,4-dihydroxyphenylalanine	-	Streptomyces globisporus	3-amino-3-(3,4-dihydroxyphenyl)propanoate	-	?
L-3-chlorotyrosine	-	Streptomyces globisporus	3-amino-3-(3-chloro-4-hydroxyphenyl)propanoate	-	?
L-tyrosine	-	Streptomyces globisporus	3-amino-3-(4-hydroxyphenyl)propanoate	-	r
L-tyrosine	involved in the biosynthesis of the enediyne antitumor antibiotic C-1027	Streptomyces globisporus	3-amino-3-(4-hydroxyphenyl)propanoate	-	?
L-tyrosine	ammonia lyase activity of tyrosine aminomutase	Streptomyces globisporus	(4-hydroxyphenyl)prop-2-enoate + NH3	-	?

Subunits

Subunits	Comment	Organism
?	x * 58100, SDS-PAGE	Streptomyces globisporus

Synonyms

Synonyms	Comment	Organism
SgcC4	-	Streptomyces globisporus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.0012	-	L-tyrosine	lyase activity	Streptomyces globisporus
0.0048	-	L-3-chlorotyrosine	-	Streptomyces globisporus
0.0067	-	L-3,4-dihydroxyphenylalanine	-	Streptomyces globisporus
0.01	-	L-tyrosine	-	Streptomyces globisporus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8.8	-	-	Streptomyces globisporus

pH Range

pH Minimum	pH Maximum	Comment	Organism
6.5	10.5	approx. 50% of maximal activity at pH 7.5 and pH 10.0, respectively	Streptomyces globisporus

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Release 2023.1 (January 2023)