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Literature summary for 5.4.3.5 extracted from

  • Maity, A.N.; Chen, Y.H.; Ke, S.C.
    Large-scale domain motions and pyridoxal-5-phosphate assisted radical catalysis in coenzyme B12-dependent aminomutases (2014), Int. J. Mol. Sci., 15, 3064-3087.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

Crystallization (Comment) Organism
crystal structure analysis Acetoanaerobium sticklandii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
D-ornithine pH and temperature not specified in the publication Acetoanaerobium sticklandii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
12800
-
2 * 12800, subunit OraS + 2 * 90000, subunit OraE, alpha2beta2 Acetoanaerobium sticklandii
90000
-
2 * 12800, subunit OraS + 2 * 90000, subunit OraE, alpha2beta2 Acetoanaerobium sticklandii
210000
-
-
Acetoanaerobium sticklandii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-ornithine Acetoanaerobium sticklandii
-
(2R,4S)-2,4-diaminopentanoate
-
r
additional information Acetoanaerobium sticklandii subunit OraS of the enzyme is capable of forming a complex with recombinant enzyme (KamDE) containing only E1 of lysine 5,6-aminomutase, EC 5.4.3.4, and restores its allosteric regulation by ATP ?
-
?

Organism

Organism UniProt Comment Textmining
Acetoanaerobium sticklandii
-
-
-

Reaction

Reaction Comment Organism Reaction ID
D-ornithine = (2R,4S)-2,4-diaminopentanoate catalytic mechanism, overview Acetoanaerobium sticklandii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-ornithine
-
Acetoanaerobium sticklandii (2R,4S)-2,4-diaminopentanoate
-
r
D-ornithine the enzyme is highly specific for D-ornithine as a substrate, substrate binding structure, overview Acetoanaerobium sticklandii (2R,4S)-2,4-diaminopentanoate
-
r
additional information subunit OraS of the enzyme is capable of forming a complex with recombinant enzyme (KamDE) containing only E1 of lysine 5,6-aminomutase, EC 5.4.3.4, and restores its allosteric regulation by ATP Acetoanaerobium sticklandii ?
-
?

Subunits

Subunits Comment Organism
tetramer 2 * 12800, subunit OraS + 2 * 90000, subunit OraE, alpha2beta2 Acetoanaerobium sticklandii

Synonyms

Synonyms Comment Organism
4,5-OAM
-
Acetoanaerobium sticklandii
ornithine 4,5-aminomutase
-
Acetoanaerobium sticklandii

Cofactor

Cofactor Comment Organism Structure
5'-deoxyadenosylcobalamin shows ability to produce highly reactive 5'-deoxyadenosyl radical in enzymatic environments Acetoanaerobium sticklandii
pyridoxal 5'-phosphate stabilizes high-energy intermediates for performing challenging 1,2-amino rearrangements between adjacent carbons, binding site structure, overview Acetoanaerobium sticklandii

General Information

General Information Comment Organism
evolution the enzyme belongs to the class III dAdoCbl-dependent isomerase family Acetoanaerobium sticklandii
additional information modeling of the closed conformation of the enzyme, domain motions, overview Acetoanaerobium sticklandii

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
15.2
-
D-ornithine pH and temperature not specified in the publication Acetoanaerobium sticklandii