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Literature summary for 5.4.3.5 extracted from
- Purification and properties of a pyridoxal phosphate and coenzyme B12 dependent D-alpha-ornithine 5,4-aminomutase (1973), Biochemistry, 12, 2597-2604.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| mercaptan | keeps essential labile sulfhydryl groups reduced and protects from oxygen | Acetoanaerobium sticklandii |  |
General Stability
| General Stability | Organism |
|---|---|
| glycerol stabilizes during storage and purification | Acetoanaerobium sticklandii |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2,4-Diaminopentanoic acid | product inhibition above 0.7 mM | Acetoanaerobium sticklandii | |
| beta-Lys | - |
Acetoanaerobium sticklandii | |
| DL-alpha-Lys | - |
Acetoanaerobium sticklandii | |
| iodoacetate | - |
Acetoanaerobium sticklandii | |
| KCl | - |
Acetoanaerobium sticklandii | |
| L-alpha-Orn | - |
Acetoanaerobium sticklandii | |
| NEM | - |
Acetoanaerobium sticklandii | |
| PCMB | - |
Acetoanaerobium sticklandii | |
| potassium phosphate | - |
Acetoanaerobium sticklandii | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.44 | - |
D-Orn | coupled assay with C4 dehydrogenase | Acetoanaerobium sticklandii | |
| 6.7 | - |
D-Orn | radiochemical assay | Acetoanaerobium sticklandii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 180000 | - |
gel electrophoresis, gel filtration, sucrose density gradient centrifugation | Acetoanaerobium sticklandii |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-Orn | Acetoanaerobium sticklandii | enzyme of ornithine fermentation | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acetoanaerobium sticklandii | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Acetoanaerobium sticklandii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 4.38 | - |
- |
Acetoanaerobium sticklandii |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, at a concentration of 0.7 mg/ml, 35% loss of activity after 1 month | Acetoanaerobium sticklandii |
| 4°C, 20% glycerol v/v or 0.02 mM coenzyme B12, enzyme concentration 1.0 mg/ml, 9% loss of activity after 2 d | Acetoanaerobium sticklandii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-Orn | r | Acetoanaerobium sticklandii | L(4S)-2,4-Diaminopentanoate | - |
? | |
| D-Orn | enzyme of ornithine fermentation | Acetoanaerobium sticklandii | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 95000-98000, SDS-PAGE | Acetoanaerobium sticklandii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Acetoanaerobium sticklandii |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 23 | 49 | half maximal activities at 23°C and 49°C | Acetoanaerobium sticklandii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
rapid inactivation above 45°C | Acetoanaerobium sticklandii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
Acetoanaerobium sticklandii |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | 9.7 | half maximal activities at pH 7.4 and 9.7 | Acetoanaerobium sticklandii |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | required, Km: 0.00036 mM | Acetoanaerobium sticklandii | |
| vitamin B12 | required | Acetoanaerobium sticklandii | |
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