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Literature summary for 5.4.3.3 extracted from

  • Tseng, C.H.; Yang, C.H.; Lin, H.J.; Wu, C.; Chen, H.P.
    The S subunit of D-ornithine aminomutase from Clostridium sticklandii is responsible for the allosteric regulation in D-alpha-lysine aminomutase (2007), FEMS Microbiol. Lett., 274, 148-153.
    View publication on PubMed
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Activating Compound

Activating Compound Comment Organism Structure
ATP protein KamDE comprised of the 30000 and 51000 Da subunits of the E1 component of D-alpha-lysine aminomutase is catalytically active in absence of the third 128000 Da subunit, but ATP no longer has a regulatory effect on it. The S subunit of D-ornithine aminomutase, OraS, is capable of forming a complex with KamDE and restores the enzyme’s ATP-dependent allosteric regulation. ATP lowers the Km value of the KamDE-OraS complex for adenosylcobalamin and pyridoxal phosphate Acetoanaerobium sticklandii

Cloned(Commentary)

Cloned (Comment) Organism
genes kamDE coding for the 30000 and 51000 Da subunits of the E1 component of D-alpha-lysine aminomutase, expression in Escherichia coli Acetoanaerobium sticklandii

Protein Variants

Protein Variants Comment Organism
additional information protein KamDE comprised of the 30000 and 51000 kDa subunits of the E1 component of D-alpha-lysine aminomutase is catalytically active in absence of the third 12800 kDa subunit, but ATP no longer has a regulatory effect on it. The S subunit of D-ornithine aminomutase, OraS, is capable of forming a complex with KamDE and restores the enzyme’s ATP-dependent allosteric regulation. OraS protein alone lowers the Km of KamDE for adenosylcobalamin and pyridoxal phosphate Acetoanaerobium sticklandii

Organism

Organism UniProt Comment Textmining
Acetoanaerobium sticklandii
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-
-

Purification (Commentary)

Purification (Comment) Organism
protein KamDE comprised of the 30000 and 51000 Da subunits of the E1 component of D-alpha-lysine aminomutase, expression in Escherichia coli Acetoanaerobium sticklandii

Storage Stability

Storage Stability Organism
-20°C, 50% glycerol, protein KamDE comprised of the 30000 and 51000 Da subunits of the E1 component of D-alpha-lysine aminomutase can be stored for several months with significant loss of activity Acetoanaerobium sticklandii

Subunits

Subunits Comment Organism
More protein KamDE comprised of the 30 and 51 kDa subunits of the E1 component of D-alpha-lysine aminomutase is catalytically active in absence of the third 12.8 kDa subunit, but ATP no longer has a regulatory effect on it. The S subunit of D-ornithine aminomutase, OraS, is capable of forming a complex with KamDE and restores the enzyme’s ATP-dependent allosteric regulation Acetoanaerobium sticklandii

Cofactor

Cofactor Comment Organism Structure
adenosylcobalamin Km value 6.1 mM, in presence of S-subunit of D-ornithine aminomutase, Km value 3.5 mM, in presence of ATP and S-subunit of D-ornithine aminomutase, Km value 1.9 mM Acetoanaerobium sticklandii
pyridoxal 5'-phosphate Km value 3.9 mM, in presence of S-subunit of D-ornithine aminomutase, Km value 3.0 mM, in presence of ATP and S-subunit of D-ornithine aminomutase, Km value 2.4 mM Acetoanaerobium sticklandii