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Literature summary for 5.4.3.2 extracted from

  • Ruzicka, F.J.; Frey, P.A.
    Kinetic and spectroscopic evidence of negative cooperativity in the action of lysine 2,3-aminomutase (2010), J. Phys. Chem. B, 114, 16118-16124.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.4.3.2

Activating Compound

Activating Compound Comment Organism Structure
ethylamine saturating ethylamine accelerates the L-alanine reaction 70fold Clostridium subterminale
methylamine saturating methylamine accelerates the L-2-aminobutyrate reaction 47fold Clostridium subterminale

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Clostridium subterminale

Organism

Organism UniProt Comment Textmining
Clostridium subterminale
-
-
-
Clostridium subterminale SB4
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Sephacryl S200 gel filtration Clostridium subterminale

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-2-aminobutyrate very low activity Clostridium subterminale L-3-aminobutyrate
-
 ?
L-2-aminobutyrate very low activity Clostridium subterminale SB4 L-3-aminobutyrate
-
 ?
L-alanine very low activity Clostridium subterminale beta-alanine
-
 ?
L-alanine very low activity Clostridium subterminale SB4 beta-alanine
-
 ?
L-lysine highly specific for L-lysine Clostridium subterminale (3S)-3,6-diaminohexanoic acid
-
 r
L-lysine highly specific for L-lysine Clostridium subterminale SB4 (3S)-3,6-diaminohexanoic acid
-
 r
additional information the enzyme does not display detectable activity toward D-lysine, L-glutamate, L-glutamine, L-threonine, L-homoserine, L-tyrosine, L-phenylalanine, L-valine, L-leucine, L-isoleucine, L-histidine, L-tryptophan, L-ornithine, or L-arginine Clostridium subterminale ?
-
 ?
additional information the enzyme does not display detectable activity toward D-lysine, L-glutamate, L-glutamine, L-threonine, L-homoserine, L-tyrosine, L-phenylalanine, L-valine, L-leucine, L-isoleucine, L-histidine, L-tryptophan, L-ornithine, or L-arginine Clostridium subterminale SB4 ?
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 000, SDS-PAGE Clostridium subterminale

Synonyms

Synonyms Comment Organism
LAM
-
 Clostridium subterminale

Cofactor

Cofactor Comment Organism Structure
4Fe-4S-center
-
 Clostridium subterminale
pyridoxal 5'-phosphate dependent on Clostridium subterminale
S-adenosyl-L-methionine
-
 Clostridium subterminale

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
8
-
 L-lysine at pH 8.0, 37°C, in 0.2 M Na-EPPS buffer Clostridium subterminale