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Literature summary for 5.4.3.11 extracted from

  • Strom, S.; Wanninayake, U.; Ratnayake, N.; Walker, K.; Geiger, J.
    Insights into the mechanistic pathway of the Pantoea agglomerans phenylalanine aminomutase (2012), Angew. Chem. Int. Ed. Engl., 51, 2898-2902.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
4-methylidene-1H-imidazol-5(4H)-one cofactor is formed autocatalytically from a Thr-Ser-Gly tandem Pantoea agglomerans

Crystallization (Commentary)

Crystallization (Comment) Organism
to 1.7 A resolution with cinnamate included in the crystallization buffer. The monomer consists of mostly alphahelices that run parallel to one another and form a four-helix bundle at the center. The catalytically relevant species is a dimer of dimers, in which the two monomers in the asymmetric unit are related by a crystallographic twofold axis to the other two monomers that comprise the catalytically functional tetramer, and each subunit contains an active site. During the reaction, the phenylpropanoid carbon backbone remains mostly stationary above the amino group that is attached to the cofactor moiety Pantoea agglomerans

Protein Variants

Protein Variants Comment Organism
F455A reaction proceeds at approximately 2% of the rate of wild-type, in a 40:60 ratio of products D-beta-phenylalanine and trans-cinnamate, where trans-cinnamate prevails Pantoea agglomerans
F455N reaction proceeds at approximately 2% of the rate of wild-type, in a 40:60 ratio of products D-beta-phenylalanine and trans-cinnamate, where trans-cinnamate prevails Pantoea agglomerans

Organism

Organism UniProt Comment Textmining
Pantoea agglomerans Q84FL5
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-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-phenylalanine
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Pantoea agglomerans D-beta-phenylalanine plus trans-cinnamate. At steady state, enzyme converts (S)-alpha-phenylalanine to (S)-beta-phenylalanine/transcinnamate in a 90:10 ratio ?
additional information enzyme reacts by an alkylamine elimination pathway, which involves covalent attachment between the amino group of the substrate and the product as well as the 4-methylidene-1H-imidazol-5(4H)-one cofactor Pantoea agglomerans ?
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?

Synonyms

Synonyms Comment Organism
admH
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Pantoea agglomerans
PAM
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Pantoea agglomerans