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Literature summary for 5.4.2.9 extracted from
- Carboxyphosphoenolpyruvate phosphonomutase, a novel enzyme catalyzing C-P bond formation (1990), J. Bacteriol., 172, 3066-3072.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | similar enzyme | Streptomyces hygroscopicus |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | increases activity | Streptomyces hygroscopicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 32000 | - |
2 * 32000, SDS-PAGE, similar enzyme | Streptomyces hygroscopicus |
| 45000 | 67000 | Streptomyces hygroscopicus SF1293, gel filtration, similar enzyme | Streptomyces hygroscopicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphoenolpyruvate | Streptomyces hygroscopicus | enzyme catalyzes the formation of a C-P bond that is involved in the biosynthesis of the antibiotic bialaphos, i.e. SF-1293 | 3-phosphonopyruvate | - |
? | |
| phosphoenolpyruvate | Streptomyces hygroscopicus SF1293 | enzyme catalyzes the formation of a C-P bond that is involved in the biosynthesis of the antibiotic bialaphos, i.e. SF-1293 | 3-phosphonopyruvate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces hygroscopicus | - |
similar enzyme | - |
| Streptomyces hygroscopicus | - |
SF1293 | - |
| Streptomyces hygroscopicus | - |
mutant NP71 | - |
| Streptomyces hygroscopicus SF1293 | - |
SF1293 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| SF1293, mutant NP71 | Streptomyces hygroscopicus |
| similar enzyme | Streptomyces hygroscopicus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Streptomyces hygroscopicus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 80% ammonium sulfate suspension, stable for 1 month, similar enzyme | Streptomyces hygroscopicus |
| 4°C, 50 mM MES buffer, pH 6.5, 1 mg protein/ml, stable for 1 week, similar enzyme | Streptomyces hygroscopicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| carboxyphosphoenolpyruvate | - |
Streptomyces hygroscopicus | phosphinopyruvate + CO2 | - |
? | |
| carboxyphosphoenolpyruvate | - |
Streptomyces hygroscopicus SF1293 | phosphinopyruvate + CO2 | - |
? | |
| phosphoenolpyruvate | enzyme catalyzes the formation of a C-P bond that is involved in the biosynthesis of the antibiotic bialaphos, i.e. SF-1293 | Streptomyces hygroscopicus | 3-phosphonopyruvate | - |
? | |
| phosphoenolpyruvate | enzyme catalyzes the formation of a C-P bond that is involved in the biosynthesis of the antibiotic bialaphos, i.e. SF-1293 | Streptomyces hygroscopicus SF1293 | 3-phosphonopyruvate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 32000, SDS-PAGE, similar enzyme | Streptomyces hygroscopicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
similar enzyme | Streptomyces hygroscopicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
similar enzyme | Streptomyces hygroscopicus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 8 | stable in this range, similar enzyme | Streptomyces hygroscopicus |
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