Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain BL21(DE3)pLysE cells | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
G7R | mutant exhibits activity similar to wild type enzyme | Arabidopsis thaliana |
G7R/R37Q | temperature-sensitive Arabidopsis thaliana PMM-12 mutant (G7R/R37Q) has lower PMM protein and enzyme activity levels than the wild type enzyme | Arabidopsis thaliana |
R37Q | mutant exhibits decreased activity similar to wild type enzyme | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.068 | - |
alpha-D-mannose 1-phosphate | mutant enzyme G7R/R37Q, at 28°C | Arabidopsis thaliana | |
0.111 | - |
alpha-D-mannose 1-phosphate | mutant enzyme R37Q, at 28°C | Arabidopsis thaliana | |
0.2 | - |
alpha-D-mannose 1-phosphate | wild type enzyme, at 28°C | Arabidopsis thaliana | |
0.204 | - |
alpha-D-mannose 1-phosphate | mutant enzyme G7R/R37Q, at 18°C | Arabidopsis thaliana | |
0.229 | - |
alpha-D-mannose 1-phosphate | mutant enzyme G7R, at 28°C | Arabidopsis thaliana | |
0.294 | - |
alpha-D-mannose 1-phosphate | mutant enzyme R37Q, at 18°C | Arabidopsis thaliana | |
0.387 | - |
alpha-D-mannose 1-phosphate | wild type enzyme, at 18°C | Arabidopsis thaliana | |
0.475 | - |
alpha-D-mannose 1-phosphate | mutant enzyme G7R, at 18°C | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Talon metal affinity resin chromatography | Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-D-mannose 1-phosphate | - |
Arabidopsis thaliana | alpha-D-mannose 6-phosphate | - |
r |
Synonyms | Comment | Organism |
---|---|---|
PMM | - |
Arabidopsis thaliana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.18 | - |
alpha-D-mannose 1-phosphate | mutant enzyme G7R/R37Q, at 18°C | Arabidopsis thaliana | |
0.24 | - |
alpha-D-mannose 1-phosphate | mutant enzyme G7R/R37Q, at 28°C | Arabidopsis thaliana | |
0.44 | - |
alpha-D-mannose 1-phosphate | mutant enzyme R37Q, at 18°C | Arabidopsis thaliana | |
0.67 | - |
alpha-D-mannose 1-phosphate | mutant enzyme R37Q, at 28°C | Arabidopsis thaliana | |
4.13 | - |
alpha-D-mannose 1-phosphate | wild type enzyme, at 18°C | Arabidopsis thaliana | |
4.18 | - |
alpha-D-mannose 1-phosphate | mutant enzyme G7R, at 18°C | Arabidopsis thaliana | |
10.01 | - |
alpha-D-mannose 1-phosphate | wild type enzyme, at 28°C | Arabidopsis thaliana | |
10.31 | - |
alpha-D-mannose 1-phosphate | mutant enzyme G7R, at 28°C | Arabidopsis thaliana |