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Literature summary for 5.4.2.8 extracted from

  • Qian, W.; Yu, C.; Qin, H.; Liu, X.; Zhang, A.; Johansen, I.E.; Wang, D.
    Molecular and functional analysis of phosphomannomutase (PMM) from higher plants and genetic evidence for the involvement of PMM in ascorbic acid biosynthesis in Arabidopsis and Nicotiana benthamiana (2007), Plant J., 49, 399-413.
    View publication on PubMed
Search for more literature for 5.4.2.8

Activating Compound

Activating Compound Comment Organism Structure
D-Glucose 1,6-bisphosphate in presence of 0.02 and 0.2 mM mannose 1-phosphate substrate, half-maximal stimulation of recombinant enzyme at 0.004 and 0.008 mM D-glucose 1,6-bisphosphate Arabidopsis thaliana

Cloned(Commentary)

Cloned (Comment) Organism
-
 Nicotiana benthamiana
expression in Escherichia coli with His-tag, expression as green fluorescent protein fusion protein in Arabidospsis thaliana Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
additional information expression as green fluorescent protein fusion protein in Arabidospsis thaliana increases ascorbic acid content by 25-33% Arabidopsis thaliana
additional information reducing enzyme expression level through virus-induced gene silencing causes substantial decrease in ascorbic acid content in leaves. Raising the expression level leads to 20-50% increase in ascorbic acid content Nicotiana benthamiana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0297
-
 D-mannose 1-phosphate 30°C, pH 7.5 Arabidopsis thaliana
0.0654
-
 D-glucose 1-phosphate 30°C, pH 7.5 Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27700
-
 x * 27700, calculated and MALDI-TOF Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana O80840
-
-
Nicotiana benthamiana
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
additional information enzyme is constitutively expressed in both vegetative and reproductive organs Arabidopsis thaliana
-
additional information enzyme is constitutively expressed in both vegetative and reproductive organs Nicotiana benthamiana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glucose 1-phosphate reaction with mannose 1-phosphate is more efficient than with D-glucose-1-phosphate Arabidopsis thaliana D-glucose 6-phosphate
-
 ?
D-glucose 1-phosphate
-
 Nicotiana benthamiana D-glucose 6-phosphate
-
 ?
D-Mannose 1-phosphate reaction with mannose 1-phosphate is more efficient than with D-glucose-1-phosphate Arabidopsis thaliana D-Mannose 6-phosphate
-
 r
D-Mannose 1-phosphate
-
 Nicotiana benthamiana D-Mannose 6-phosphate
-
 r
D-mannose 6-phosphate
-
 Arabidopsis thaliana D-mannose 1-phosphate
-
 r
D-mannose 6-phosphate
-
 Nicotiana benthamiana D-mannose 1-phosphate
-
 r

Subunits

Subunits Comment Organism
? x * 27700, calculated and MALDI-TOF Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.1
-
 95% of maximum activity Arabidopsis thaliana
7.5
-
-
 Arabidopsis thaliana