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Literature summary for 5.4.2.8 extracted from

  • Rashid, N.; Kanai, T.; Atomi, H.; Imanaka, T.
    Among multiple phosphomannomutase gene orthologues, only one gene encodes a protein with phosphoglucomutase and phosphomannomutase activities in Thermococcus kodakaraensis (2004), J. Bacteriol., 186, 6070-6076.
    View publication on PubMed
Search for more literature for 5.4.2.8

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Thermococcus kodakarensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3
-
 D-glucose-1-phosphate 90°C Thermococcus kodakarensis
3.2
-
 D-mannose-1-phosphate 90°C Thermococcus kodakarensis
3.5
-
 2-deoxyribose-1-phosphate 90°C Thermococcus kodakarensis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Thermococcus kodakarensis
additional information Ni2+, Mn2+ and Zn2+ at a concentration of 1 mM can also support activity, although to a lesser extent Thermococcus kodakarensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49850
-
 4 * 49850, protein encoded by gene TK1108, calculated Thermococcus kodakarensis
210000
-
 gel filtration Thermococcus kodakarensis

Organism

Organism UniProt Comment Textmining
Thermococcus kodakarensis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
heat treatment 85°C, 20 min, anion-exchange, hydrophobic and gel filtration column chromatography Thermococcus kodakarensis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 D-mannose-1-phosphate Vmax 0.401, 90°C Thermococcus kodakarensis
additional information
-
 D-glucose-1-phosphate Vmax 0.690, 90°C, pH 7.0 Thermococcus kodakarensis
additional information
-
 2-deoxyribose-1-phosphate Vmax 0.230, 90°C Thermococcus kodakarensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-deoxyribose 1-phosphate
-
 Thermococcus kodakarensis 2-deoxyribose 5-phosphate
-
 ?
3-phospho-D-glyceric acid no mutase activity Thermococcus kodakarensis ?
-
 ?
D-fructose-1-phosphate no mutase activity Thermococcus kodakarensis D-fructose-6-phosphate
-
 ?
D-glucosamine-1-phosphate low enzyme activity Thermococcus kodakarensis D-glucosamine-6-phosphate
-
 ?
D-glucose-1-phosphate bifunctional enzyme Thermococcus kodakarensis D-glucose-6-phosphate
-
 ?
D-mannose-1-phosphate bifunctional enzyme Thermococcus kodakarensis D-mannose-6-phosphate
-
 ?
N-acetyl-D-glucosamine-1-phosphate no mutase activity Thermococcus kodakarensis N-acetyl-D-glucosamine-6-phosphate
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 49850, protein encoded by gene TK1108, calculated Thermococcus kodakarensis

Synonyms

Synonyms Comment Organism
PMM/PGM
-
 Thermococcus kodakarensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
90
-
-
 Thermococcus kodakarensis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
100
-
 highly stable, half-life of about 85 min in boiling water in presence of 10mM Mg2+ Thermococcus kodakarensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 10mM Mg2+, 90°C Thermococcus kodakarensis