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Literature summary for 5.4.2.8 extracted from
- Purification and some properties of phosphomannomutase from corms of Amorphophallus konjac C. Koch (1976), Plant Cell Physiol., 17, 1099-1109.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| alpha-D-glucose 1,6-diphosphate | required | Amorphophallus konjac |  |
General Stability
| General Stability | Organism |
|---|---|
| Mg2+ stabilizes | Amorphophallus konjac |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Be2+ | in histidine buffer | Amorphophallus konjac | |
| Co2+ | weak | Amorphophallus konjac | |
| Cu2+ | in Tris buffer, no inhibition in histidine buffer | Amorphophallus konjac | |
| mannose 1-phosphate | at high concentrations, competitive with respect to glucose-1,6-diphosphate, Ki: 1.2 mM | Amorphophallus konjac | |
| Ni2+ | weak | Amorphophallus konjac | |
| Zn2+ | in Tris buffer, no inhibition in histidine buffer | Amorphophallus konjac | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.2 | - |
mannose 1-phosphate | - |
Amorphophallus konjac | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | 72% of the activation relative to Mg2+, maximal activity at 0.5 mM | Amorphophallus konjac | |
| Mg2+ | required | Amorphophallus konjac | |
| Mg2+ | maximal activity at 1 mM | Amorphophallus konjac | |
| Ni2+ | 36% of the activation relative to Mg2+, maximal activity at 0.5 mM | Amorphophallus konjac | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 62000 | - |
gel filtration | Amorphophallus konjac |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Amorphophallus konjac | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Amorphophallus konjac |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| corm | - |
Amorphophallus konjac | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 6.105 | - |
- |
Amorphophallus konjac |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, stable for at least 2 months | Amorphophallus konjac |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-Mannose 1-phosphate | - |
Amorphophallus konjac | D-Mannose 6-phosphate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Amorphophallus konjac |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 38 | - |
50% loss of activity within a few min, completely protected from heat inactivation by 1 mM mannose 1-phosphate, 0.01 mM glucose 1,6-diphosphate or 1 mM fructose 1,6-diphosphate. In presence of 1 mM Mg2+: 50% loss of activity after 17 min | Amorphophallus konjac |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 7 | - |
Amorphophallus konjac |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
maximal stability | Amorphophallus konjac |
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