Activating Compound | Comment | Organism | Structure |
---|---|---|---|
D-Glucose 1,6-bisphosphate | there is a concentration-dependent enhancement of PPM activity between 0.0001 mM and 0.0025 mM D-glucose 1,6-bisphosphate | Bacillus cereus |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21-Gold (DE3) cells | Bacillus cereus |
Crystallization (Comment) | Organism |
---|---|
enzyme alone, co-crystallized with ribose 5-phosphate, co-crystallized with glucose 1,6-bisphosphate, and following activation with glucose 1,6-bisphosphate, hanging drop vapor diffusion method, using 100 mM Bis-Tris, pH 5.5, 50 mM MnCl2, 14% (w/v) polyethylene glycol 3350, and 75 mM NH4CH3COO | Bacillus cereus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.263 | - |
D-ribose 5-phosphate | in 25 mM Tris-HCl, pH 8.0, and 0.1 mM MnCl2, 0.0005 mM glucose 1,6-bisphosphate,at 23°C | Bacillus cereus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | only Mn2+-incorporated enzyme shows significant enzyme activity | Bacillus cereus | |
additional information | not dependent on Zn2+, Ni2+, Mg2+, and Co2+ | Bacillus cereus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44000 | - |
x * 44000, SDS-PAGE | Bacillus cereus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus cereus | Q818Z9 | - |
- |
Bacillus cereus ATCC 14579 | Q818Z9 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | protein phosphorylation activates the enzyme | Bacillus cereus |
Purification (Comment) | Organism |
---|---|
- |
Bacillus cereus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-D-ribose 1-phosphate | - |
Bacillus cereus | D-ribose 5-phosphate | - |
r | |
alpha-D-ribose 1-phosphate | - |
Bacillus cereus ATCC 14579 | D-ribose 5-phosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 44000, SDS-PAGE | Bacillus cereus |
Synonyms | Comment | Organism |
---|---|---|
PPM | - |
Bacillus cereus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
10.2 | - |
D-ribose 5-phosphate | in 25 mM Tris-HCl, pH 8.0, and 0.1 mM MnCl2, 0.0005 mM glucose 1,6-bisphosphate,at 23°C | Bacillus cereus |