Literature summary for 5.4.2.7 extracted from

Panosian, T.D.; Nannemann, D.P.; Bachmann, B.O.; Iverson, T.M.
Crystallization and preliminary X-ray analysis of a phosphopentomutase from Bacillus cereus (2010), Acta Crystallogr. Sect. F, 66, 811-814.

Search for more literature for 5.4.2.7

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21-Gold (DE3) cells	Bacillus cereus

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop vapor diffusion method, using 100 mM Bis-Tris pH 5.5, 50 mM MnCl2, 13-16% PEG 3350, and 25-100 mM NH4CH3COO	Bacillus cereus
hanging drop vapor diffusion method, using 100 mM Bis-Tris pH 5.5, 50 mM MnCl2, 13-16% PEG 3350, and 25-100 mM NH4CH3COO	Bacillus cereus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
44000	-	x * 44000, SDS-PAGE	Bacillus cereus

Organism

Organism	UniProt	Comment	Textmining
Bacillus cereus	-	-	-
Bacillus cereus ATCC 14579D	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
HisTrap HP column chromatography and Superdex 200 gel filtration	Bacillus cereus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
alpha-D-ribose 1-phosphate	-	Bacillus cereus	D-ribose 5-phosphate	-	r
alpha-D-ribose 1-phosphate	-	Bacillus cereus ATCC 14579D	D-ribose 5-phosphate	-	r

Subunits

Subunits	Comment	Organism
?	x * 44000, SDS-PAGE	Bacillus cereus

Synonyms

Synonyms	Comment	Organism
PPM	-	Bacillus cereus

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Release 2023.1 (January 2023)