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Literature summary for 5.4.2.7 extracted from

  • Barsky, D.L.; Hoffee, P.A.
    Purification and characterization of phosphopentomutase from rat liver (1983), Biochim. Biophys. Acta, 743, 162-171.
    View publication on PubMed
Search for more literature for 5.4.2.7

Activating Compound

Activating Compound Comment Organism Structure
Cys activates Rattus norvegicus
glucose 1,6-diphosphate activates, optimal concentration 0.01 mM Rattus norvegicus

General Stability

General Stability Organism
glucose 1,6-diphosphate protects against thermal inactivation Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
AsO43-
-
 Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.083
-
 deoxyribose 1-phosphate
-
 Rattus norvegicus
0.13
-
 ribose 1-phosphate
-
 Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ stimulates Rattus norvegicus
Mg2+ optimal concentration 0.2 mM Rattus norvegicus
Mn2+ stimulates, optimal concentration is 0.2 mM Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32500
-
 2 * 32500, SDS-PAGE Rattus norvegicus
65000
-
 disc gel electrophoresis Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Rattus norvegicus

Storage Stability

Storage Stability Organism
4°C, Tris buffer or phosphate buffer, pH 7-8, stable for 1 month Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-ribose 1-phosphate
-
 Rattus norvegicus D-ribose 5-phosphate
-
 ?
Deoxyribose 1-phosphate at 25% of the activity relative to ribose 1-phosphate Rattus norvegicus Deoxyribose 5-phosphate
-
 ?
Glucose 1-phosphate at 2.4% of the activity relative to ribose 1-phosphate Rattus norvegicus ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 32500, SDS-PAGE Rattus norvegicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 pH 8, rapid loss of activity in absence of glucose 1,6-diphosphate, 70% loss of activity after 10 min, complete loss of activity after 1 h. Completely stable for 90 min in presence of 11 mM glucose 1,6-diphosphate Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8.5
-
 Rattus norvegicus