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Literature summary for 5.4.2.6 extracted from

  • Cruz-Navarrete, F.A.; Baxter, N.J.; Wood, H.P.; Hounslow, A.M.; Waltho, J.P.
    1H, 15N and 13C backbone resonance assignments of the P146A variant of beta-phosphoglucomutase from Lactococcus lactis in its substrate-free form (2019), Biomol. NMR Assign., 13, 349-356.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
beta-D-glucose 1,6-bisphosphate
-
Lactococcus lactis subsp. lactis

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Lactococcus lactis subsp. lactis

Protein Variants

Protein Variants Comment Organism
P146A the enzymatically active mutant adopta a trans K145-A146 peptide bond as the dominant population within the D137-A147 loop Lactococcus lactis subsp. lactis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ dependent on Lactococcus lactis subsp. lactis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
beta-D-Glucose 1-phosphate Lactococcus lactis subsp. lactis
-
beta-D-Glucose 6-phosphate
-
r

Organism

Organism UniProt Comment Textmining
Lactococcus lactis subsp. lactis P71447
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-Sepharose column chromatography and Superdex 75 gel filtration Lactococcus lactis subsp. lactis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-D-Glucose 1-phosphate
-
Lactococcus lactis subsp. lactis beta-D-Glucose 6-phosphate
-
r

Synonyms

Synonyms Comment Organism
betaPGM
-
Lactococcus lactis subsp. lactis
pgmB
-
Lactococcus lactis subsp. lactis