Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 5.4.2.6 extracted from

  • Jung, J.H.; Seo, D.H.; Holden, J.F.; Park, C.S.
    Identification and characterization of an archaeal kojibiose catabolic pathway in the hyperthermophilic Pyrococcus sp. strain ST04 (2014), J. Bacteriol., 196, 1122-1131.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pyrococcus sp.
heterologous expression in Escherichia coli Pyrococcus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.08
-
beta-D-glucose 1-phosphate pH 6.0, 90°C Pyrococcus sp.
2.08
-
beta-D-glucose 1-phosphate 90°C, pH not specified in the publication Pyrococcus sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ conversion of beta-D-glucose 1-phosphate into beta-D-glucose 6-phosphate in the presence of 6 mM MgCl2 Pyrococcus sp.
Mg2+ no activity without Mg2+. 6 mM is the optimal concentration Pyrococcus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000
-
x * 25000, SDS-PAGE Pyrococcus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
beta-D-Glucose 1-phosphate Pyrococcus sp. the enzyme is involved in conversion of kojibiose into beta-D-glucose 6-phosphate, a substrate of the glycolytic pathway. Disaccharide utilization pathway via phosphorolysis in hyperthermophilic archaea beta-D-Glucose 6-phosphate
-
r
beta-D-Glucose 1-phosphate Pyrococcus sp. the enzyme is involved in the kojibiose catabolic pathway beta-D-Glucose 6-phosphate
-
r
beta-D-Glucose 1-phosphate Pyrococcus sp. ST04 the enzyme is involved in conversion of kojibiose into beta-D-glucose 6-phosphate, a substrate of the glycolytic pathway. Disaccharide utilization pathway via phosphorolysis in hyperthermophilic archaea beta-D-Glucose 6-phosphate
-
r
beta-D-Glucose 1-phosphate Pyrococcus sp. ST04 the enzyme is involved in the kojibiose catabolic pathway beta-D-Glucose 6-phosphate
-
r

Organism

Organism UniProt Comment Textmining
Pyrococcus sp. I3RFR5
-
-
Pyrococcus sp. ST04 I3RFR5
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Pyrococcus sp.
recombinant enzyme Pyrococcus sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.51
-
90°C, pH not specified in the publication, conversion of beta-D-glucose 6-phosphate into beta-D-glucose 1-phosphate Pyrococcus sp.
3.51
-
pH 6.0, 90°C, conversion from beta-D-glucose 6-phosphate to beta-D-glucose 1-phosphate Pyrococcus sp.
46.81
-
90°C, pH not specified in the publication, conversion of beta-D-glucose 1-phosphate into beta-D-glucose 6-phosphate Pyrococcus sp.
46.81
-
pH 6.0, 90°C, conversion from beta-D-glucose 1-phosphate to beta-D-glucose 6-phosphate Pyrococcus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-D-Glucose 1-phosphate
-
Pyrococcus sp. beta-D-Glucose 6-phosphate
-
r
beta-D-Glucose 1-phosphate the enzyme is involved in conversion of kojibiose into beta-D-glucose 6-phosphate, a substrate of the glycolytic pathway. Disaccharide utilization pathway via phosphorolysis in hyperthermophilic archaea Pyrococcus sp. beta-D-Glucose 6-phosphate
-
r
beta-D-Glucose 1-phosphate the enzyme is involved in the kojibiose catabolic pathway Pyrococcus sp. beta-D-Glucose 6-phosphate
-
r
beta-D-Glucose 1-phosphate conversion activity from beta-D-glucose 1-phosphate to beta-D-glucose 6-phosphate is 46.81 U/mg, and reverse conversion activity from beta-D-glucose 6-phosphate to beta-D-glucose 1-phosphate is 3.51 U/mg Pyrococcus sp. beta-D-Glucose 6-phosphate
-
r
beta-D-Glucose 1-phosphate
-
Pyrococcus sp. ST04 beta-D-Glucose 6-phosphate
-
r
beta-D-Glucose 1-phosphate the enzyme is involved in conversion of kojibiose into beta-D-glucose 6-phosphate, a substrate of the glycolytic pathway. Disaccharide utilization pathway via phosphorolysis in hyperthermophilic archaea Pyrococcus sp. ST04 beta-D-Glucose 6-phosphate
-
r
beta-D-Glucose 1-phosphate the enzyme is involved in the kojibiose catabolic pathway Pyrococcus sp. ST04 beta-D-Glucose 6-phosphate
-
r
beta-D-Glucose 1-phosphate conversion activity from beta-D-glucose 1-phosphate to beta-D-glucose 6-phosphate is 46.81 U/mg, and reverse conversion activity from beta-D-glucose 6-phosphate to beta-D-glucose 1-phosphate is 3.51 U/mg Pyrococcus sp. ST04 beta-D-Glucose 6-phosphate
-
r

Subunits

Subunits Comment Organism
? x * 25000, SDS-PAGE Pyrococcus sp.

Synonyms

Synonyms Comment Organism
beta-phosphoglucomutase
-
Pyrococcus sp.
PsPGM
-
Pyrococcus sp.
Py04_1503 locus name Pyrococcus sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
95
-
-
Pyrococcus sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
20 min, stable Pyrococcus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Pyrococcus sp.

pH Range

pH Minimum pH Maximum Comment Organism
4 9
-
Pyrococcus sp.

General Information

General Information Comment Organism
physiological function the enzyme is involved in conversion of kojibiose into beta-D-glucose 6-phosphate, a substrate of the glycolytic pathway. Disaccharide utilization pathway via phosphorolysis in hyperthermophilic archaea Pyrococcus sp.
physiological function the enzyme is involved in the kojibiose catabolic pathway Pyrococcus sp.

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
9.07
-
beta-D-glucose 1-phosphate 90°C, pH not specified in the publication Pyrococcus sp.
9.07
-
beta-D-glucose 1-phosphate pH 6.0, 90°C Pyrococcus sp.