Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pyrococcus sp. |
heterologous expression in Escherichia coli | Pyrococcus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.08 | - |
beta-D-glucose 1-phosphate | pH 6.0, 90°C | Pyrococcus sp. | |
2.08 | - |
beta-D-glucose 1-phosphate | 90°C, pH not specified in the publication | Pyrococcus sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | conversion of beta-D-glucose 1-phosphate into beta-D-glucose 6-phosphate in the presence of 6 mM MgCl2 | Pyrococcus sp. | |
Mg2+ | no activity without Mg2+. 6 mM is the optimal concentration | Pyrococcus sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25000 | - |
x * 25000, SDS-PAGE | Pyrococcus sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-D-Glucose 1-phosphate | Pyrococcus sp. | the enzyme is involved in conversion of kojibiose into beta-D-glucose 6-phosphate, a substrate of the glycolytic pathway. Disaccharide utilization pathway via phosphorolysis in hyperthermophilic archaea | beta-D-Glucose 6-phosphate | - |
r | |
beta-D-Glucose 1-phosphate | Pyrococcus sp. | the enzyme is involved in the kojibiose catabolic pathway | beta-D-Glucose 6-phosphate | - |
r | |
beta-D-Glucose 1-phosphate | Pyrococcus sp. ST04 | the enzyme is involved in conversion of kojibiose into beta-D-glucose 6-phosphate, a substrate of the glycolytic pathway. Disaccharide utilization pathway via phosphorolysis in hyperthermophilic archaea | beta-D-Glucose 6-phosphate | - |
r | |
beta-D-Glucose 1-phosphate | Pyrococcus sp. ST04 | the enzyme is involved in the kojibiose catabolic pathway | beta-D-Glucose 6-phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus sp. | I3RFR5 | - |
- |
Pyrococcus sp. ST04 | I3RFR5 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Pyrococcus sp. |
recombinant enzyme | Pyrococcus sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.51 | - |
90°C, pH not specified in the publication, conversion of beta-D-glucose 6-phosphate into beta-D-glucose 1-phosphate | Pyrococcus sp. |
3.51 | - |
pH 6.0, 90°C, conversion from beta-D-glucose 6-phosphate to beta-D-glucose 1-phosphate | Pyrococcus sp. |
46.81 | - |
90°C, pH not specified in the publication, conversion of beta-D-glucose 1-phosphate into beta-D-glucose 6-phosphate | Pyrococcus sp. |
46.81 | - |
pH 6.0, 90°C, conversion from beta-D-glucose 1-phosphate to beta-D-glucose 6-phosphate | Pyrococcus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-D-Glucose 1-phosphate | - |
Pyrococcus sp. | beta-D-Glucose 6-phosphate | - |
r | |
beta-D-Glucose 1-phosphate | the enzyme is involved in conversion of kojibiose into beta-D-glucose 6-phosphate, a substrate of the glycolytic pathway. Disaccharide utilization pathway via phosphorolysis in hyperthermophilic archaea | Pyrococcus sp. | beta-D-Glucose 6-phosphate | - |
r | |
beta-D-Glucose 1-phosphate | the enzyme is involved in the kojibiose catabolic pathway | Pyrococcus sp. | beta-D-Glucose 6-phosphate | - |
r | |
beta-D-Glucose 1-phosphate | conversion activity from beta-D-glucose 1-phosphate to beta-D-glucose 6-phosphate is 46.81 U/mg, and reverse conversion activity from beta-D-glucose 6-phosphate to beta-D-glucose 1-phosphate is 3.51 U/mg | Pyrococcus sp. | beta-D-Glucose 6-phosphate | - |
r | |
beta-D-Glucose 1-phosphate | - |
Pyrococcus sp. ST04 | beta-D-Glucose 6-phosphate | - |
r | |
beta-D-Glucose 1-phosphate | the enzyme is involved in conversion of kojibiose into beta-D-glucose 6-phosphate, a substrate of the glycolytic pathway. Disaccharide utilization pathway via phosphorolysis in hyperthermophilic archaea | Pyrococcus sp. ST04 | beta-D-Glucose 6-phosphate | - |
r | |
beta-D-Glucose 1-phosphate | the enzyme is involved in the kojibiose catabolic pathway | Pyrococcus sp. ST04 | beta-D-Glucose 6-phosphate | - |
r | |
beta-D-Glucose 1-phosphate | conversion activity from beta-D-glucose 1-phosphate to beta-D-glucose 6-phosphate is 46.81 U/mg, and reverse conversion activity from beta-D-glucose 6-phosphate to beta-D-glucose 1-phosphate is 3.51 U/mg | Pyrococcus sp. ST04 | beta-D-Glucose 6-phosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 25000, SDS-PAGE | Pyrococcus sp. |
Synonyms | Comment | Organism |
---|---|---|
beta-phosphoglucomutase | - |
Pyrococcus sp. |
PsPGM | - |
Pyrococcus sp. |
Py04_1503 | locus name | Pyrococcus sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
95 | - |
- |
Pyrococcus sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
20 min, stable | Pyrococcus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Pyrococcus sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 9 | - |
Pyrococcus sp. |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is involved in conversion of kojibiose into beta-D-glucose 6-phosphate, a substrate of the glycolytic pathway. Disaccharide utilization pathway via phosphorolysis in hyperthermophilic archaea | Pyrococcus sp. |
physiological function | the enzyme is involved in the kojibiose catabolic pathway | Pyrococcus sp. |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
9.07 | - |
beta-D-glucose 1-phosphate | 90°C, pH not specified in the publication | Pyrococcus sp. | |
9.07 | - |
beta-D-glucose 1-phosphate | pH 6.0, 90°C | Pyrococcus sp. |