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Literature summary for 5.4.2.6 extracted from
- Purification and enzymatic characterization of PgcM: A beta-phosphoglucomutase and glucose-1-phosphate phosphodismutase of Bacillus subtilis (2000), Arch. Microbiol., 174, 256-264.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| beta-D-glucose 1,6-bisphosphate | - |
Bacillus subtilis |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression in Bacillus mergaterium | Bacillus subtilis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.004 | - |
beta-D-glucose 1-phosphate | phosphoglucomutase activity | Bacillus subtilis | |
| 0.006 | - |
beta-D-glucose 1-phosphate | 1-phosphate phosphodismutase activity | Bacillus subtilis | |
| 0.021 | - |
alpha-D-glucose 1-phosphate | 1-phosphate phosphodismutase activity | Bacillus subtilis | |
| 0.027 | - |
alpha-D-glucose 1-phosphate | phosphoglucomutase activity | Bacillus subtilis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required for activity, maximal activity at 10 mM | Bacillus subtilis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 26500 | - |
3 * 26500, SDS-PAGE | Bacillus subtilis |
| 80000 | - |
nonreducing SDS-PAGE | Bacillus subtilis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beta-D-Glucose 1-phosphate | Bacillus subtilis | - |
beta-D-Glucose 6-phosphate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant PgcM | Bacillus subtilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-D-glucose 1-phosphate | 7fold lower affinity than with beta-D-glucose | Bacillus subtilis | alpha-D-glucose 6-phosphate | - |
r | |
| beta-D-Glucose 1-phosphate | - |
Bacillus subtilis | beta-D-Glucose 6-phosphate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| trimer | 3 * 26500, SDS-PAGE | Bacillus subtilis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PgcM | - |
Bacillus subtilis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 95 | - |
no loss of activity after 30 min | Bacillus subtilis |
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