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Literature summary for 5.4.2.6 extracted from
- Purification and some properties of beta-phosphoglucomutase from Lactobacillus lactis subsp. cremonis IFP 3427 (1998), J. Ferment. Bioeng., 85, 350-353.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | - |
Lactobacillus delbrueckii subsp. lactis |  |
| ADP | strong inhibition | Lactococcus lactis | |
| AgNO3 | - |
Lactobacillus delbrueckii subsp. lactis | |
| alpha-D-glucose 1-phosphate | 10 mM, 12% inhibition | Lactobacillus delbrueckii subsp. lactis | |
| AMP | 10 mM, weak | Lactobacillus delbrueckii subsp. lactis | |
| ATP | - |
Lactobacillus delbrueckii subsp. lactis | |
| ATP | strong inhibition | Lactococcus lactis | |
| CaCl2 | 1 mM, 77% inhibition | Lactobacillus delbrueckii subsp. lactis | |
| Cd2+ | - |
Lactobacillus delbrueckii subsp. lactis | |
| Cd2+ | strong inhibition | Lactococcus lactis | |
| Cu2+ | - |
Lactobacillus delbrueckii subsp. lactis | |
| Cu2+ | strong inhibition | Lactococcus lactis | |
| D-fructose 1-6-diphosphate | 10 mM, 63% inhibition | Lactobacillus delbrueckii subsp. lactis | |
| D-glucose 6-phosphate | 10 mM, 11% inhibition | Lactobacillus delbrueckii subsp. lactis | |
| Fe2+ | 1 mM FeCl2, 68% inhibition | Lactobacillus delbrueckii subsp. lactis | |
| Hg2+ | - |
Lactobacillus delbrueckii subsp. lactis | |
| Hg2+ | strong inhibition | Lactococcus lactis | |
| Zn2+ | - |
Lactobacillus delbrueckii subsp. lactis | |
| Zn2+ | strong inhibition | Lactococcus lactis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.23 | - |
beta-D-glucose 1-phosphate | - |
Lactobacillus delbrueckii subsp. lactis | |
| 0.23 | - |
beta-D-glucose 1-phosphate | - |
Lactococcus lactis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | activated by divalent cations at 1 mM in decreasing order: Co2+, Mn2+, Mg2+, Ni2+ | Lactobacillus delbrueckii subsp. lactis | |
| Co2+ | activation at 1 mM, efficiency of activation in descending order, Co2+, Mn2+, Mg2+ and Ni2+ | Lactococcus lactis | |
| Mg2+ | activated by divalent cations at 1 mM in decreasing order: Co2+, Mn2+, Mg2+, Ni2+ | Lactobacillus delbrueckii subsp. lactis | |
| Mg2+ | activation at 1 mM, efficiency of activation in descending order, Co2+, Mn2+, Mg2+ and Ni2+ | Lactococcus lactis | |
| Mn2+ | activated by divalent cations at 1 mM in decreasing order: Co2+, Mn2+, Mg2+, Ni2+ | Lactobacillus delbrueckii subsp. lactis | |
| Mn2+ | activation at 1 mM, efficiency of activation in descending order, Co2+, Mn2+, Mg2+ and Ni2+ | Lactococcus lactis | |
| Ni2+ | activated by divalent cations at 1 mM in decreasing order: Co2+, Mn2+, Mg2+, Ni2+ | Lactobacillus delbrueckii subsp. lactis | |
| Ni2+ | activation at 1 mM, efficiency of activation in descending order, Co2+, Mn2+, Mg2+ and Ni2+ | Lactococcus lactis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 25000 | - |
1 * 25000, SDS-PAGE | Lactococcus lactis |
| 25000 | - |
x * 25000, SDS-PAGE | Lactobacillus delbrueckii subsp. lactis |
| 34000 | - |
gel filtration | Lactobacillus delbrueckii subsp. lactis |
| 34000 | - |
gel filtration | Lactococcus lactis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beta-D-Glucose 1-phosphate | Lactococcus lactis | - |
beta-D-Glucose 6-phosphate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lactobacillus delbrueckii subsp. lactis | - |
subsp. cremonis | - |
| Lactococcus lactis | - |
subspecies cremoris IFO 3427 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Lactobacillus delbrueckii subsp. lactis |
| QAE-Sephadex A-50, Phenyl-Sepharose, hydroxylapatite, Bio-Gel A-1.5m | Lactococcus lactis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 113 | - |
- |
Lactobacillus delbrueckii subsp. lactis |
| 113 | - |
- |
Lactococcus lactis |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 37°C, stable for 1 week | Lactobacillus delbrueckii subsp. lactis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beta-D-Glucose 1-phosphate | - |
Lactococcus lactis | beta-D-Glucose 6-phosphate | - |
r | |
| beta-D-Glucose 1-phosphate | - |
Lactobacillus delbrueckii subsp. lactis | beta-D-Glucose 6-phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | x * 25000, SDS-PAGE | Lactobacillus delbrueckii subsp. lactis |
| monomer | 1 * 25000, SDS-PAGE | Lactococcus lactis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| beta-PGM | - |
Lactococcus lactis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Lactobacillus delbrueckii subsp. lactis |
| 40 | - |
- |
Lactococcus lactis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 50 | 25°C: about 70% of maximal activity, 50°C: about 40% of maximal activity | Lactobacillus delbrueckii subsp. lactis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
30 min, pH 5.0-9.5 | Lactobacillus delbrueckii subsp. lactis |
| 37 | - |
stable for 1 week | Lactobacillus delbrueckii subsp. lactis |
| 45 | - |
pH 7.0, 15 min, stable up to | Lactobacillus delbrueckii subsp. lactis |
| 45 | - |
no loss of activity | Lactococcus lactis |
| 50 | - |
15 min, 12% loss of activity | Lactobacillus delbrueckii subsp. lactis |
| 60 | - |
15 min, 60% loss of activity | Lactobacillus delbrueckii subsp. lactis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Lactobacillus delbrueckii subsp. lactis |
| 7 | - |
- |
Lactococcus lactis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9.5 | - |
Lactococcus lactis |
| 6 | 8 | pH 6: about 30% of maximal activity, pH 8.0: about 50% of maximal activity | Lactobacillus delbrueckii subsp. lactis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9.5 | 30°C, 30 min, stable | Lactobacillus delbrueckii subsp. lactis |
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