BRENDA - Enzyme Database
show all sequences of 5.4.2.5

D-Glucose-1-phosphate:D-glucose-6-phosphotransferase

Fujimoto, A.; Ingram, P.; Smith, R.A.; Biochim. Biophys. Acta 96, 91-101 (1965)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
additional information
no effect on the rate of reaction found for cysteine, glutathione, 2-mercaptoethanol, glucose 1,6-diphosphate, ribose 1,5-diphosphate
Mycolicibacterium smegmatis
Inhibitors
Inhibitors
Commentary
Organism
Structure
F-
strong inhibitor
Mycolicibacterium smegmatis
iodoacetate
-
Mycolicibacterium smegmatis
additional information
no inhibition by NaN3
Mycolicibacterium smegmatis
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.9
-
alpha-D-glucose 1-phosphate
direct assay with unreacted glucose 1-phosphate
Escherichia coli
1.1
-
alpha-D-glucose 1-phosphate
assay coupled with glucose-6-phosphate dehydrogenase, following NADPH formation
Escherichia coli
8.5
-
alpha-D-glucose
assay coupled with glucose-6-phosphate dehydrogenase, following NADPH formation
Escherichia coli
17
-
alpha-D-glucose
direct assay with unreacted glucose 1-phosphate
Escherichia coli
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
additional information
no requirement for Mg2+, Mn2+, Fe2+
Mycolicibacterium smegmatis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
alpha-D-Glucose 1-phosphate + D-glucose
Escherichia coli
-
?
-
-
-
alpha-D-Glucose 1-phosphate + D-glucose
Mycolicibacterium smegmatis
-
?
-
-
-
alpha-D-Glucose 1-phosphate + D-glucose
Klebsiella aerogenes
-
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
-
-
-
Klebsiella aerogenes
-
-
-
Mycolicibacterium smegmatis
-
-
-
Purification (Commentary)
Commentary
Organism
-
Mycolicibacterium smegmatis
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
114
-
-
Mycolicibacterium smegmatis
Storage Stability
Storage Stability
Organism
-15°C, 3 years stable
Mycolicibacterium smegmatis
0°C, 3 months stable
Mycolicibacterium smegmatis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-Deoxy-D-glucose + alpha-D-glucose 1-phosphate
-
3330
Escherichia coli
D-Glucose + 2-deoxy-D-glucose 6-phosphate
-
-
-
-
3-O-Methyl-D-glucose + alpha-D-glucose 1-phosphate
-
3330
Escherichia coli
3-O-Methyl-D-glucose 6-phosphate + D-glucose
-
-
-
-
alpha-D-Glucose 1-phosphate + D-glucose
-
3330
Escherichia coli
D-Glucose + alpha-D-glucose 6-phosphate
-
3330
Escherichia coli
-
alpha-D-Glucose 1-phosphate + D-glucose
-
3330
Mycolicibacterium smegmatis
D-Glucose + alpha-D-glucose 6-phosphate
-
-
-
-
alpha-D-Glucose 1-phosphate + D-glucose
-
3330
Klebsiella aerogenes
D-Glucose + alpha-D-glucose 6-phosphate
-
-
-
-
alpha-D-Glucose 1-phosphate + D-glucose
-
3330
Escherichia coli
?
-
-
-
-
alpha-D-Glucose 1-phosphate + D-glucose
-
3330
Mycolicibacterium smegmatis
?
-
-
-
-
alpha-D-Glucose 1-phosphate + D-glucose
-
3330
Klebsiella aerogenes
?
-
-
-
-
alpha-Methyl-D-glucoside + alpha-D-glucose 1-phosphate
-
3330
Escherichia coli
alpha-Methyl-D-glucoside 6-phosphate + D-glucose
-
-
-
-
beta-Methyl-D-glucoside + alpha-D-glucose 1-phosphate
-
3330
Escherichia coli
beta-Methyl-D-glucoside 6-phosphate + D-glucose
-
-
-
-
D-Fructose + alpha-D-glucose 1-phosphate
-
3330
Escherichia coli
D-Fructose 6-phosphate + D-glucose
-
3330
Escherichia coli
-
D-Galactose + alpha-D-glucose 1-phosphate
-
3330
Escherichia coli
D-Galactose 6-phosphate + D-glucose
-
-
-
-
D-Mannose + alpha-D-glucose 1-phosphate
-
3330
Escherichia coli
D-Mannose 6-phosphate + D-glucose
-
3330
Escherichia coli
-
Fructose 1-phosphate + D-glucose
-
3330
Escherichia coli
D-glucose 6-phosphate + fructose
-
3330
Escherichia coli
-
L-Sorbose + alpha-D-glucose 1-phosphate
-
3330
Escherichia coli
L-Sorbose 6-phosphate + D-glucose
-
-
-
-
additional information
no substrates are 6-deoxy-D-glucose, D-ribose, D-xylose, DL-serine, cytidine, adenosine, uridine, guanosine
3330
Escherichia coli
?
-
-
-
-
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5
-
direct assay with unreacted glucose 1-phosphate
Mycolicibacterium smegmatis
6.5
-
assay coupled with glucose-6-phosphate dehydrogenase, following NADPH formation
Mycolicibacterium smegmatis
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
additional information
no effect on the rate of reaction found for cysteine, glutathione, 2-mercaptoethanol, glucose 1,6-diphosphate, ribose 1,5-diphosphate
Mycolicibacterium smegmatis
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
F-
strong inhibitor
Mycolicibacterium smegmatis
iodoacetate
-
Mycolicibacterium smegmatis
additional information
no inhibition by NaN3
Mycolicibacterium smegmatis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.9
-
alpha-D-glucose 1-phosphate
direct assay with unreacted glucose 1-phosphate
Escherichia coli
1.1
-
alpha-D-glucose 1-phosphate
assay coupled with glucose-6-phosphate dehydrogenase, following NADPH formation
Escherichia coli
8.5
-
alpha-D-glucose
assay coupled with glucose-6-phosphate dehydrogenase, following NADPH formation
Escherichia coli
17
-
alpha-D-glucose
direct assay with unreacted glucose 1-phosphate
Escherichia coli
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
additional information
no requirement for Mg2+, Mn2+, Fe2+
Mycolicibacterium smegmatis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
alpha-D-Glucose 1-phosphate + D-glucose
Escherichia coli
-
?
-
-
-
alpha-D-Glucose 1-phosphate + D-glucose
Mycolicibacterium smegmatis
-
?
-
-
-
alpha-D-Glucose 1-phosphate + D-glucose
Klebsiella aerogenes
-
?
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
-
Mycolicibacterium smegmatis
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
114
-
-
Mycolicibacterium smegmatis
Storage Stability (protein specific)
Storage Stability
Organism
-15°C, 3 years stable
Mycolicibacterium smegmatis
0°C, 3 months stable
Mycolicibacterium smegmatis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-Deoxy-D-glucose + alpha-D-glucose 1-phosphate
-
3330
Escherichia coli
D-Glucose + 2-deoxy-D-glucose 6-phosphate
-
-
-
-
3-O-Methyl-D-glucose + alpha-D-glucose 1-phosphate
-
3330
Escherichia coli
3-O-Methyl-D-glucose 6-phosphate + D-glucose
-
-
-
-
alpha-D-Glucose 1-phosphate + D-glucose
-
3330
Escherichia coli
D-Glucose + alpha-D-glucose 6-phosphate
-
3330
Escherichia coli
-
alpha-D-Glucose 1-phosphate + D-glucose
-
3330
Mycolicibacterium smegmatis
D-Glucose + alpha-D-glucose 6-phosphate
-
-
-
-
alpha-D-Glucose 1-phosphate + D-glucose
-
3330
Klebsiella aerogenes
D-Glucose + alpha-D-glucose 6-phosphate
-
-
-
-
alpha-D-Glucose 1-phosphate + D-glucose
-
3330
Escherichia coli
?
-
-
-
-
alpha-D-Glucose 1-phosphate + D-glucose
-
3330
Mycolicibacterium smegmatis
?
-
-
-
-
alpha-D-Glucose 1-phosphate + D-glucose
-
3330
Klebsiella aerogenes
?
-
-
-
-
alpha-Methyl-D-glucoside + alpha-D-glucose 1-phosphate
-
3330
Escherichia coli
alpha-Methyl-D-glucoside 6-phosphate + D-glucose
-
-
-
-
beta-Methyl-D-glucoside + alpha-D-glucose 1-phosphate
-
3330
Escherichia coli
beta-Methyl-D-glucoside 6-phosphate + D-glucose
-
-
-
-
D-Fructose + alpha-D-glucose 1-phosphate
-
3330
Escherichia coli
D-Fructose 6-phosphate + D-glucose
-
3330
Escherichia coli
-
D-Galactose + alpha-D-glucose 1-phosphate
-
3330
Escherichia coli
D-Galactose 6-phosphate + D-glucose
-
-
-
-
D-Mannose + alpha-D-glucose 1-phosphate
-
3330
Escherichia coli
D-Mannose 6-phosphate + D-glucose
-
3330
Escherichia coli
-
Fructose 1-phosphate + D-glucose
-
3330
Escherichia coli
D-glucose 6-phosphate + fructose
-
3330
Escherichia coli
-
L-Sorbose + alpha-D-glucose 1-phosphate
-
3330
Escherichia coli
L-Sorbose 6-phosphate + D-glucose
-
-
-
-
additional information
no substrates are 6-deoxy-D-glucose, D-ribose, D-xylose, DL-serine, cytidine, adenosine, uridine, guanosine
3330
Escherichia coli
?
-
-
-
-
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5
-
direct assay with unreacted glucose 1-phosphate
Mycolicibacterium smegmatis
6.5
-
assay coupled with glucose-6-phosphate dehydrogenase, following NADPH formation
Mycolicibacterium smegmatis
Other publictions for EC 5.4.2.5
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
3330
Fujimoto
D-Glucose-1-phosphate:D-glucos ...
Escherichia coli, Klebsiella aerogenes, Mycolicibacterium smegmatis
Biochim. Biophys. Acta
96
91-101
1965
1
-
-
-
-
-
3
4
-
1
-
3
-
3
-
-
1
-
-
-
1
2
16
-
-
-
-
-
2
-
-
-
-
-
-
1
-
-
-
-
-
-
-
3
-
4
-
1
-
3
-
-
-
1
-
-
1
2
16
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-