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Literature summary for 5.4.2.3 extracted from

  • Fernandez-Sorensen, A.; Carlson, D.M.
    Purification and properties of phosphoglucosamine mutase (1971), J. Biol. Chem., 246, 3485-3493.
    View publication on PubMed
Search for more literature for 5.4.2.3

Activating Compound

Activating Compound Comment Organism Structure
8-hydroxyquinoline metal chelator required Sus scrofa
Cys metal chelator required Sus scrofa
diethyldithiocarbamate metal chelator required Sus scrofa
fructose 1,6-diphosphate or glucose 1,6-diphosphate activates, Km: 0.018 mM Sus scrofa
glucose 1,6-diphosphate or fructose 1,6-diphosphate activates, Km: 0.002 mM Sus scrofa
sulfhydryl compound increases activity Sus scrofa

General Stability

General Stability Organism
purified enzyme loses more than 90% activity upon one freezing and thawing Sus scrofa

Inhibitors

Inhibitors Comment Organism Structure
Be2+ 0.02 mM in presence of 1 mM Mg2+, complete inhibition Sus scrofa
Cd2+ 0.5 mM in presence of 1 mM Mg2+, complete inhibition Sus scrofa
Cu2+ 0.5 mM in presence of 1 mM Mg2+, complete inhibition Sus scrofa
Mn2+ 1 mM in presence of 1 mM Mg2+, complete inhibition Sus scrofa
Zn2+ 0.5 mM in presence of 1 mM Mg2+, complete inhibition Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.29
-
 N-acetylglucosamine 1-phosphate
-
 Sus scrofa
0.39
-
 glucose 1-phosphate
-
 Sus scrofa

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ divalent metal ion required. Optimal Mg2+ concentration in the presence of dithiothreitol is 10 mM, maximal activity with other metal chelators is obtained with 1 mM Mg2+ Sus scrofa
Mn2+ divalent metal ion required. Mn2+ is less efficient than Mg2+ Sus scrofa

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
submandibular gland
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Sus scrofa

Storage Stability

Storage Stability Organism
0°C, 30% sucrose or 30% glycerol, 50% loss of activity after 3 weeks. Addition of albumin or varying the pH does not appreciably enhance the stability of the enzyme Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Glucose 1-phosphate
-
 Sus scrofa Glucose 6-phosphate
-
 ?
N-Acetyl-D-glucosamine 1-phosphate
-
 Sus scrofa N-Acetyl-D-glucosamine 6-phosphate
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7 Tris-HCl buffer Sus scrofa
7 7.5 Tris-maleate buffer Sus scrofa

pH Range

pH Minimum pH Maximum Comment Organism
6.5 9 pH 6.5: about 45% of maximal activity, pH 9.0: about 40% of maximal activity, Tris-maleate buffer Sus scrofa