Cloned (Comment) | Organism |
---|---|
cloned and homologously overexpressed in Lactococcus lactis subsp. cremoris strain NZ9000, introducing 6xHis-tag | Lactococcus lactis |
Crystallization (Comment) | Organism |
---|---|
crystallized with vapor diffusion, streak seeding, in ammonium sulfate solution, optimized crystals diffract to 1.5 A resolution | Lactococcus lactis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29880 | - |
calculated | Lactococcus lactis |
30000 | - |
SDS-PAGE of purified alpha-PGM, with His-tag | Lactococcus lactis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactococcus lactis | Q00G41 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein is purified by centrifugation und affinity chromatography with Ni-NTA Superflow cartridge. Purity is assessed by SDS-PAGE | Lactococcus lactis |
Storage Stability | Organism |
---|---|
-80°C, fast-frozen in LN2 and stored for 2 years without affecting the sample homogeneity | Lactococcus lactis |
Synonyms | Comment | Organism |
---|---|---|
alpha-Pgm | - |
Lactococcus lactis |
alpha-phosphoglucomutase | - |
Lactococcus lactis |
General Information | Comment | Organism |
---|---|---|
physiological function | Unlike the majority of alpha-phosphoglucomutases, the described enzyme of 252 residues with alpha-phosphoglucomutase activity, is related to eukaryotic phosphomannomutases and belongs to HAD-superfamily hydrolase, subfamily IIB. | Lactococcus lactis |