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Literature summary for 5.4.2.2 extracted from

  • Wang, Y.; Zhang, Y.H.
    A highly active phosphoglucomutase from Clostridium thermocellum: cloning, purification, characterization and enhanced thermostability (2010), J. Appl. Microbiol., 108, 39-46.
    View publication on PubMed
Search for more literature for 5.4.2.2

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Acetivibrio thermocellus

General Stability

General Stability Organism
at pH 7.5, the PGM activity in the HEPES buffer is 20% lower than in the Tris-HCl buffer Acetivibrio thermocellus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.41
-
 alpha-D-glucose 1-phosphate recombinant wild type enzyme, at 60°C Acetivibrio thermocellus
0.44
-
 alpha-D-mannose 1-phosphate recombinant wild type enzyme, at 60°C Acetivibrio thermocellus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ activator Acetivibrio thermocellus
Mn2+ activator Acetivibrio thermocellus
additional information Zn2+, Cu2+ and Pd2+ do not enhance the PGM activity Acetivibrio thermocellus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
64900
-
 estimated from amino acid sequence Acetivibrio thermocellus
65000
-
 gel filtration Acetivibrio thermocellus

Organism

Organism UniProt Comment Textmining
Acetivibrio thermocellus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ultracentrifugation Acetivibrio thermocellus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-D-glucose 1-phosphate
-
 Acetivibrio thermocellus alpha-D-glucose 6-phosphate
-
 ?
alpha-D-mannose 1-phosphate
-
 Acetivibrio thermocellus alpha-D-mannose 6-phosphate
-
 ?
additional information PGM from Clostridium thermocellum is a dual-specificity enzyme with phosphoglucomutase and phosphomannomutase activities Acetivibrio thermocellus ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 65000, gel filtration Acetivibrio thermocellus

Synonyms

Synonyms Comment Organism
PGM
-
 Acetivibrio thermocellus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
 Acetivibrio thermocellus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
50 80
-
 Acetivibrio thermocellus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60 70 at 60°C PGM is relatively stable and loses 10% of its initial activity after incubation for 8 h. At 70°C, it deactivates quickly with a half-life of 0.8 h. PGM at a concentration of 500 nM has a half-life of 30 h at 60°C, while 2 nM or 50 nM PGM loses nearly 100% of activity within 12 h. Thermostability of PGM at a low concentration (2 nM, 100 units/l) is enhanced by 12fold at 60°C with addition of 1 mg/ml bovine serum albumin, 0.1% (v/v) Triton X-100, 5 mM Mg2+, and 0.5 mM Mn2+ Acetivibrio thermocellus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
59
-
 alpha-D-mannose 1-phosphate recombinant wild type enzyme, at 60°C Acetivibrio thermocellus
190
-
 alpha-D-glucose 1-phosphate recombinant wild type enzyme, at 60°C Acetivibrio thermocellus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.8
-
 around pH 8.8 Acetivibrio thermocellus