Cloned (Comment) | Organism |
---|---|
overexpression of histidine-tagged fusion protein in Escherichia coli | Saccharolobus solfataricus |
Protein Variants | Comment | Organism |
---|---|---|
S309A | activity of mutant enzyme with D-glucose 6-phosphate is 58% compared to activity of wild-type enzyme | Saccharolobus solfataricus |
S309D | activity of mutant enzyme with D-glucose 6-phosphate is 4% compared to activity of wild-type enzyme | Saccharolobus solfataricus |
S309N | activity of mutant enzyme with D-glucose 6-phosphate is 27% compared to activity of wild-type enzyme | Saccharolobus solfataricus |
S309Q | activity of mutant enzyme with D-glucose 6-phosphate is 22% compared to activity of wild-type enzyme | Saccharolobus solfataricus |
S309T | activity of mutant enzyme with D-glucose 6-phosphate is 36% compared to activity of wild-type enzyme | Saccharolobus solfataricus |
S309V | activity of mutant enzyme with D-glucose 6-phosphate is 36% compared to activity of wild-type enzyme | Saccharolobus solfataricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | - |
D-glucose 6-phosphate | pH 6.5, 65°C, mutant enzyme S309D | Saccharolobus solfataricus | |
6 | - |
D-glucose 6-phosphate | pH 6.5, 65°C, mutant enzyme S309Q | Saccharolobus solfataricus | |
8 | - |
D-glucose 6-phosphate | pH 6.5, 65°C, mutant enzyme S309N | Saccharolobus solfataricus | |
8 | - |
D-glucose 6-phosphate | pH 6.5, 65°C, mutant enzyme S309T | Saccharolobus solfataricus | |
9 | - |
D-glucose 6-phosphate | pH 6.5, 65°C, mutant enzyme S309A | Saccharolobus solfataricus | |
12 | - |
D-glucose 6-phosphate | pH 6.5, 65°C, mutant enzyme S309V | Saccharolobus solfataricus | |
13 | - |
D-glucose 6-phosphate | pH 6.5, 65°C, wild-type enzyme | Saccharolobus solfataricus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | divalent metal required, preferentially Mn2+ or Mg2+ | Saccharolobus solfataricus | |
Mn2+ | divalent metal required, preferentially Mn2+ or Mg2+ | Saccharolobus solfataricus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-D-glucose 1-phosphate | Saccharolobus solfataricus | it is unclear whether either glucose or mannose phosphate represents the cellular substrate for SsoPHM. The Km values for rSsoPHM toward glucose 6-phosphate and mannose 6-phosphate are in the low millimolar range, significantly higher than that expected for a physiological substrate. Since the protein encoded by sso0207 is the only identifiable phosphohexomutase in the Sulfolbus solfataricus P2 genome, SsoPHM provides the only recognizable avenue for interconverting glucose 6-phosphate and glucose 1-phosphate during glycogen synthesis and breakdown | D-glucose 6-phosphate | - |
r | |
alpha-D-glucose 1-phosphate | Saccharolobus solfataricus P2 | it is unclear whether either glucose or mannose phosphate represents the cellular substrate for SsoPHM. The Km values for rSsoPHM toward glucose 6-phosphate and mannose 6-phosphate are in the low millimolar range, significantly higher than that expected for a physiological substrate. Since the protein encoded by sso0207 is the only identifiable phosphohexomutase in the Sulfolbus solfataricus P2 genome, SsoPHM provides the only recognizable avenue for interconverting glucose 6-phosphate and glucose 1-phosphate during glycogen synthesis and breakdown | D-glucose 6-phosphate | - |
r | |
D-glucose 6-phosphate | Saccharolobus solfataricus | it is unclear whether either glucose or mannose phosphate represents the cellular substrate for SsoPHM. The Km values for rSsoPHM toward glucose 6-phosphate and mannose 6-phosphate are in the low millimolar range, significantly higher than that expected for a physiological substrate. Since the protein encoded by sso0207 is the only identifiable phosphohexomutase in the Sulfolbus solfataricus P2 genome, SsoPHM provides the only recognizable avenue for interconverting glucose 6-phosphate and glucose 1-phosphate during glycogen synthesis and breakdown | alpha-D-glucose 1-phosphate | - |
r | |
D-glucose 6-phosphate | Saccharolobus solfataricus P2 | it is unclear whether either glucose or mannose phosphate represents the cellular substrate for SsoPHM. The Km values for rSsoPHM toward glucose 6-phosphate and mannose 6-phosphate are in the low millimolar range, significantly higher than that expected for a physiological substrate. Since the protein encoded by sso0207 is the only identifiable phosphohexomutase in the Sulfolbus solfataricus P2 genome, SsoPHM provides the only recognizable avenue for interconverting glucose 6-phosphate and glucose 1-phosphate during glycogen synthesis and breakdown | alpha-D-glucose 1-phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | Q980S1 | - |
- |
Saccharolobus solfataricus P2 | Q980S1 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharolobus solfataricus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0026 | - |
pH 6.5, 65°C | Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-D-glucose 1-phosphate | it is unclear whether either glucose or mannose phosphate represents the cellular substrate for SsoPHM. The Km values for rSsoPHM toward glucose 6-phosphate and mannose 6-phosphate are in the low millimolar range, significantly higher than that expected for a physiological substrate. Since the protein encoded by sso0207 is the only identifiable phosphohexomutase in the Sulfolbus solfataricus P2 genome, SsoPHM provides the only recognizable avenue for interconverting glucose 6-phosphate and glucose 1-phosphate during glycogen synthesis and breakdown | Saccharolobus solfataricus | D-glucose 6-phosphate | - |
r | |
alpha-D-glucose 1-phosphate | the enzyme also catalyzes the interconversion of alpha-D-mannose 1-phosphate and D-mannose 6-phosphate | Saccharolobus solfataricus | D-glucose 6-phosphate | - |
r | |
alpha-D-glucose 1-phosphate | it is unclear whether either glucose or mannose phosphate represents the cellular substrate for SsoPHM. The Km values for rSsoPHM toward glucose 6-phosphate and mannose 6-phosphate are in the low millimolar range, significantly higher than that expected for a physiological substrate. Since the protein encoded by sso0207 is the only identifiable phosphohexomutase in the Sulfolbus solfataricus P2 genome, SsoPHM provides the only recognizable avenue for interconverting glucose 6-phosphate and glucose 1-phosphate during glycogen synthesis and breakdown | Saccharolobus solfataricus P2 | D-glucose 6-phosphate | - |
r | |
alpha-D-glucose 1-phosphate | the enzyme also catalyzes the interconversion of alpha-D-mannose 1-phosphate and D-mannose 6-phosphate | Saccharolobus solfataricus P2 | D-glucose 6-phosphate | - |
r | |
D-glucose 6-phosphate | it is unclear whether either glucose or mannose phosphate represents the cellular substrate for SsoPHM. The Km values for rSsoPHM toward glucose 6-phosphate and mannose 6-phosphate are in the low millimolar range, significantly higher than that expected for a physiological substrate. Since the protein encoded by sso0207 is the only identifiable phosphohexomutase in the Sulfolbus solfataricus P2 genome, SsoPHM provides the only recognizable avenue for interconverting glucose 6-phosphate and glucose 1-phosphate during glycogen synthesis and breakdown | Saccharolobus solfataricus | alpha-D-glucose 1-phosphate | - |
r | |
D-glucose 6-phosphate | the enzyme also catalyzes the interconversion of alpha-D-mannose 1-phosphate and D-mannose 6-phosphate. No activity toward glucosamine 6-phosphate or N-acetylglucosamine 6-phosphate | Saccharolobus solfataricus | alpha-D-glucose 1-phosphate | - |
r | |
D-glucose 6-phosphate | it is unclear whether either glucose or mannose phosphate represents the cellular substrate for SsoPHM. The Km values for rSsoPHM toward glucose 6-phosphate and mannose 6-phosphate are in the low millimolar range, significantly higher than that expected for a physiological substrate. Since the protein encoded by sso0207 is the only identifiable phosphohexomutase in the Sulfolbus solfataricus P2 genome, SsoPHM provides the only recognizable avenue for interconverting glucose 6-phosphate and glucose 1-phosphate during glycogen synthesis and breakdown | Saccharolobus solfataricus P2 | alpha-D-glucose 1-phosphate | - |
r | |
D-glucose 6-phosphate | the enzyme also catalyzes the interconversion of alpha-D-mannose 1-phosphate and D-mannose 6-phosphate. No activity toward glucosamine 6-phosphate or N-acetylglucosamine 6-phosphate | Saccharolobus solfataricus P2 | alpha-D-glucose 1-phosphate | - |
r |
Synonyms | Comment | Organism |
---|---|---|
Phosphohexomutase | - |
Saccharolobus solfataricus |
SSO0207 | locus name | Saccharolobus solfataricus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
assay at | Saccharolobus solfataricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
assay at | Saccharolobus solfataricus |