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Literature summary for 5.4.2.2 extracted from

  • Ray, W.K.; Keith, S.M.; DeSantis, A.M.; Hunt, J.P.; Larson, T.J.; Helm, R.F.; Kennelly, P.J.
    A phosphohexomutase from the archaeon Sulfolobus solfataricus is covalently modified by phosphorylation on serine (2005), J. Bacteriol., 187, 4270-4275.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of histidine-tagged fusion protein in Escherichia coli Saccharolobus solfataricus

Protein Variants

Protein Variants Comment Organism
S309A activity of mutant enzyme with D-glucose 6-phosphate is 58% compared to activity of wild-type enzyme Saccharolobus solfataricus
S309D activity of mutant enzyme with D-glucose 6-phosphate is 4% compared to activity of wild-type enzyme Saccharolobus solfataricus
S309N activity of mutant enzyme with D-glucose 6-phosphate is 27% compared to activity of wild-type enzyme Saccharolobus solfataricus
S309Q activity of mutant enzyme with D-glucose 6-phosphate is 22% compared to activity of wild-type enzyme Saccharolobus solfataricus
S309T activity of mutant enzyme with D-glucose 6-phosphate is 36% compared to activity of wild-type enzyme Saccharolobus solfataricus
S309V activity of mutant enzyme with D-glucose 6-phosphate is 36% compared to activity of wild-type enzyme Saccharolobus solfataricus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2
-
D-glucose 6-phosphate pH 6.5, 65°C, mutant enzyme S309D Saccharolobus solfataricus
6
-
D-glucose 6-phosphate pH 6.5, 65°C, mutant enzyme S309Q Saccharolobus solfataricus
8
-
D-glucose 6-phosphate pH 6.5, 65°C, mutant enzyme S309N Saccharolobus solfataricus
8
-
D-glucose 6-phosphate pH 6.5, 65°C, mutant enzyme S309T Saccharolobus solfataricus
9
-
D-glucose 6-phosphate pH 6.5, 65°C, mutant enzyme S309A Saccharolobus solfataricus
12
-
D-glucose 6-phosphate pH 6.5, 65°C, mutant enzyme S309V Saccharolobus solfataricus
13
-
D-glucose 6-phosphate pH 6.5, 65°C, wild-type enzyme Saccharolobus solfataricus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ divalent metal required, preferentially Mn2+ or Mg2+ Saccharolobus solfataricus
Mn2+ divalent metal required, preferentially Mn2+ or Mg2+ Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
alpha-D-glucose 1-phosphate Saccharolobus solfataricus it is unclear whether either glucose or mannose phosphate represents the cellular substrate for SsoPHM. The Km values for rSsoPHM toward glucose 6-phosphate and mannose 6-phosphate are in the low millimolar range, significantly higher than that expected for a physiological substrate. Since the protein encoded by sso0207 is the only identifiable phosphohexomutase in the Sulfolbus solfataricus P2 genome, SsoPHM provides the only recognizable avenue for interconverting glucose 6-phosphate and glucose 1-phosphate during glycogen synthesis and breakdown D-glucose 6-phosphate
-
r
alpha-D-glucose 1-phosphate Saccharolobus solfataricus P2 it is unclear whether either glucose or mannose phosphate represents the cellular substrate for SsoPHM. The Km values for rSsoPHM toward glucose 6-phosphate and mannose 6-phosphate are in the low millimolar range, significantly higher than that expected for a physiological substrate. Since the protein encoded by sso0207 is the only identifiable phosphohexomutase in the Sulfolbus solfataricus P2 genome, SsoPHM provides the only recognizable avenue for interconverting glucose 6-phosphate and glucose 1-phosphate during glycogen synthesis and breakdown D-glucose 6-phosphate
-
r
D-glucose 6-phosphate Saccharolobus solfataricus it is unclear whether either glucose or mannose phosphate represents the cellular substrate for SsoPHM. The Km values for rSsoPHM toward glucose 6-phosphate and mannose 6-phosphate are in the low millimolar range, significantly higher than that expected for a physiological substrate. Since the protein encoded by sso0207 is the only identifiable phosphohexomutase in the Sulfolbus solfataricus P2 genome, SsoPHM provides the only recognizable avenue for interconverting glucose 6-phosphate and glucose 1-phosphate during glycogen synthesis and breakdown alpha-D-glucose 1-phosphate
-
r
D-glucose 6-phosphate Saccharolobus solfataricus P2 it is unclear whether either glucose or mannose phosphate represents the cellular substrate for SsoPHM. The Km values for rSsoPHM toward glucose 6-phosphate and mannose 6-phosphate are in the low millimolar range, significantly higher than that expected for a physiological substrate. Since the protein encoded by sso0207 is the only identifiable phosphohexomutase in the Sulfolbus solfataricus P2 genome, SsoPHM provides the only recognizable avenue for interconverting glucose 6-phosphate and glucose 1-phosphate during glycogen synthesis and breakdown alpha-D-glucose 1-phosphate
-
r

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus Q980S1
-
-
Saccharolobus solfataricus P2 Q980S1
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Saccharolobus solfataricus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0026
-
pH 6.5, 65°C Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-D-glucose 1-phosphate it is unclear whether either glucose or mannose phosphate represents the cellular substrate for SsoPHM. The Km values for rSsoPHM toward glucose 6-phosphate and mannose 6-phosphate are in the low millimolar range, significantly higher than that expected for a physiological substrate. Since the protein encoded by sso0207 is the only identifiable phosphohexomutase in the Sulfolbus solfataricus P2 genome, SsoPHM provides the only recognizable avenue for interconverting glucose 6-phosphate and glucose 1-phosphate during glycogen synthesis and breakdown Saccharolobus solfataricus D-glucose 6-phosphate
-
r
alpha-D-glucose 1-phosphate the enzyme also catalyzes the interconversion of alpha-D-mannose 1-phosphate and D-mannose 6-phosphate Saccharolobus solfataricus D-glucose 6-phosphate
-
r
alpha-D-glucose 1-phosphate it is unclear whether either glucose or mannose phosphate represents the cellular substrate for SsoPHM. The Km values for rSsoPHM toward glucose 6-phosphate and mannose 6-phosphate are in the low millimolar range, significantly higher than that expected for a physiological substrate. Since the protein encoded by sso0207 is the only identifiable phosphohexomutase in the Sulfolbus solfataricus P2 genome, SsoPHM provides the only recognizable avenue for interconverting glucose 6-phosphate and glucose 1-phosphate during glycogen synthesis and breakdown Saccharolobus solfataricus P2 D-glucose 6-phosphate
-
r
alpha-D-glucose 1-phosphate the enzyme also catalyzes the interconversion of alpha-D-mannose 1-phosphate and D-mannose 6-phosphate Saccharolobus solfataricus P2 D-glucose 6-phosphate
-
r
D-glucose 6-phosphate it is unclear whether either glucose or mannose phosphate represents the cellular substrate for SsoPHM. The Km values for rSsoPHM toward glucose 6-phosphate and mannose 6-phosphate are in the low millimolar range, significantly higher than that expected for a physiological substrate. Since the protein encoded by sso0207 is the only identifiable phosphohexomutase in the Sulfolbus solfataricus P2 genome, SsoPHM provides the only recognizable avenue for interconverting glucose 6-phosphate and glucose 1-phosphate during glycogen synthesis and breakdown Saccharolobus solfataricus alpha-D-glucose 1-phosphate
-
r
D-glucose 6-phosphate the enzyme also catalyzes the interconversion of alpha-D-mannose 1-phosphate and D-mannose 6-phosphate. No activity toward glucosamine 6-phosphate or N-acetylglucosamine 6-phosphate Saccharolobus solfataricus alpha-D-glucose 1-phosphate
-
r
D-glucose 6-phosphate it is unclear whether either glucose or mannose phosphate represents the cellular substrate for SsoPHM. The Km values for rSsoPHM toward glucose 6-phosphate and mannose 6-phosphate are in the low millimolar range, significantly higher than that expected for a physiological substrate. Since the protein encoded by sso0207 is the only identifiable phosphohexomutase in the Sulfolbus solfataricus P2 genome, SsoPHM provides the only recognizable avenue for interconverting glucose 6-phosphate and glucose 1-phosphate during glycogen synthesis and breakdown Saccharolobus solfataricus P2 alpha-D-glucose 1-phosphate
-
r
D-glucose 6-phosphate the enzyme also catalyzes the interconversion of alpha-D-mannose 1-phosphate and D-mannose 6-phosphate. No activity toward glucosamine 6-phosphate or N-acetylglucosamine 6-phosphate Saccharolobus solfataricus P2 alpha-D-glucose 1-phosphate
-
r

Synonyms

Synonyms Comment Organism
Phosphohexomutase
-
Saccharolobus solfataricus
SSO0207 locus name Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
assay at Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
assay at Saccharolobus solfataricus