Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pyrococcus horikoshii |
Protein Variants | Comment | Organism |
---|---|---|
S101A | mutant protein PH0923S101A to investigate the role of the serine residue | Pyrococcus horikoshii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | KM mannose-1-phosphate isoenzyme PH0923S101A not detectable, 37°C | Pyrococcus horikoshii | |
0.0915 | - |
D-mannose-1-phosphate | isoenzyme PH0923, 37°C | Pyrococcus horikoshii | |
0.0952 | - |
D-glucose-1-phosphate | isoenzyme PH0923, 65°C | Pyrococcus horikoshii | |
0.127 | - |
D-glucose-1-phosphate | isoenzyme PH0923S101A, 65°C | Pyrococcus horikoshii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 1% relative activity | Pyrococcus horikoshii | |
Co2+ | 44% relative activity | Pyrococcus horikoshii | |
Mg2+ | 100% relative activity | Pyrococcus horikoshii | |
Mn2+ | 20% relative activity | Pyrococcus horikoshii | |
additional information | no detectable activity after addition of 0.1 mM EDTA | Pyrococcus horikoshii | |
Ni2+ | 15% relative activity | Pyrococcus horikoshii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
SDS-PAGE | Pyrococcus horikoshii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus horikoshii | - |
OT3 | - |
Pyrococcus horikoshii OT-3 | - |
OT3 | - |
Purification (Comment) | Organism |
---|---|
nickel-affinity column chromatography | Pyrococcus horikoshii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
isoenzyme PH0923 PGM Vmax 24, 65°C | Pyrococcus horikoshii |
additional information | - |
isoenzyme PH0923 PMM Vmax 0.7, 37°C | Pyrococcus horikoshii |
additional information | - |
isoenzyme PH0923S101A PGM Vmax 0.4, 65°C | Pyrococcus horikoshii |
additional information | - |
isoenzyme PH0923S101A PMM Vmax not detectable, 37°C | Pyrococcus horikoshii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose-1-phosphate | - |
Pyrococcus horikoshii | D-glucose-6-phosphate | - |
r | |
D-glucose-1-phosphate | - |
Pyrococcus horikoshii OT-3 | D-glucose-6-phosphate | - |
r | |
D-mannose-1-phosphate | - |
Pyrococcus horikoshii | D-mannose-6-phosphate | - |
r | |
D-mannose-1-phosphate | - |
Pyrococcus horikoshii OT-3 | D-mannose-6-phosphate | - |
r |
Synonyms | Comment | Organism |
---|---|---|
PMM/PGM | bifunctional enzyme | Pyrococcus horikoshii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
95 | - |
longer than 90 min stable | Pyrococcus horikoshii |
100 | - |
50% activity after 5 min | Pyrococcus horikoshii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Pyrococcus horikoshii |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 10 | - |
Pyrococcus horikoshii |