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Literature summary for 5.4.2.2 extracted from
- Characterization of a thermostable enzyme with phosphomannomutase/phosphoglucomutase activities from the hyperthermophilic archaeon Pyrococcus horikoshii OT3 (2005), J. Biochem., 138, 159-166.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Pyrococcus horikoshii |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| S101A | mutant protein PH0923S101A to investigate the role of the serine residue | Pyrococcus horikoshii |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | KM mannose-1-phosphate isoenzyme PH0923S101A not detectable, 37°C | Pyrococcus horikoshii | |
| 0.0915 | - |
D-mannose-1-phosphate | isoenzyme PH0923, 37°C | Pyrococcus horikoshii |  |
| 0.0952 | - |
D-glucose-1-phosphate | isoenzyme PH0923, 65°C | Pyrococcus horikoshii | |
| 0.127 | - |
D-glucose-1-phosphate | isoenzyme PH0923S101A, 65°C | Pyrococcus horikoshii | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 1% relative activity | Pyrococcus horikoshii | |
| Co2+ | 44% relative activity | Pyrococcus horikoshii | |
| Mg2+ | 100% relative activity | Pyrococcus horikoshii | |
| Mn2+ | 20% relative activity | Pyrococcus horikoshii | |
| additional information | no detectable activity after addition of 0.1 mM EDTA | Pyrococcus horikoshii | |
| Ni2+ | 15% relative activity | Pyrococcus horikoshii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
SDS-PAGE | Pyrococcus horikoshii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pyrococcus horikoshii | - |
OT3 | - |
| Pyrococcus horikoshii OT-3 | - |
OT3 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| nickel-affinity column chromatography | Pyrococcus horikoshii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
isoenzyme PH0923 PMM Vmax 0.7, 37°C | Pyrococcus horikoshii |
| additional information | - |
isoenzyme PH0923 PGM Vmax 24, 65°C | Pyrococcus horikoshii |
| additional information | - |
isoenzyme PH0923S101A PMM Vmax not detectable, 37°C | Pyrococcus horikoshii |
| additional information | - |
isoenzyme PH0923S101A PGM Vmax 0.4, 65°C | Pyrococcus horikoshii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glucose-1-phosphate | - |
Pyrococcus horikoshii | D-glucose-6-phosphate | - |
r | |
| D-glucose-1-phosphate | - |
Pyrococcus horikoshii OT-3 | D-glucose-6-phosphate | - |
r | |
| D-mannose-1-phosphate | - |
Pyrococcus horikoshii | D-mannose-6-phosphate | - |
r | |
| D-mannose-1-phosphate | - |
Pyrococcus horikoshii OT-3 | D-mannose-6-phosphate | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PMM/PGM | bifunctional enzyme | Pyrococcus horikoshii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 95 | - |
longer than 90 min stable | Pyrococcus horikoshii |
| 100 | - |
50% activity after 5 min | Pyrococcus horikoshii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
Pyrococcus horikoshii |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 10 | - |
Pyrococcus horikoshii |
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