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Literature summary for 5.4.2.2 extracted from
- Purification, separation and characterization of phosphoglucomutase and phosphomannomutase from maize leaves (1998), Biochem. Mol. Biol. Int., 46, 461-470.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| D-glucose-1,6-bisphosphate | - |
Zea mays |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| D-Glucose 1,6-bisphosphate | - |
Zea mays | |
| D-glucose 1-phosphate | - |
Zea mays | |
| D-mannose 1-phosphate | - |
Zea mays | |
| Mn2+ | approx. 50% inhibition at 5 mM, almost complete inhibition at 7 mM | Zea mays | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.016 | - |
alpha-D-Glucose 1,6-bisphosphate | 25°C, pH 7.6 | Zea mays | |
| 0.02 | - |
alpha-D-glucose 1-phosphate | 25°C, pH 7.6 | Zea mays | |
| 0.02 | - |
D-mannose 1-phosphate | 25°C, pH 7.6 | Zea mays | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | approx. 4fold activation at 4 mM | Zea mays | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 66000 | - |
2 * 66000, SDS-PAGE | Zea mays |
| 132000 | - |
gel filtration | Zea mays |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-D-glucose 1-phosphate | Zea mays | - |
alpha-D-glucose 6-phosphate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Zea mays | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate, Sephadex G-25, DEAE-Toyopearl, Toyopearl HW-65 | Zea mays |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 11.7 | - |
- |
Zea mays |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 0-4°C, 50% glycerol, 2-3 d, no loss of activity | Zea mays |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-D-glucose 1,6-bisphosphate + H2O | - |
Zea mays | alpha-D-glucose 6-phosphate + phosphate | - |
? | |
| alpha-D-glucose 1-phosphate | - |
Zea mays | alpha-D-glucose 6-phosphate | - |
r | |
| alpha-D-glucose 1-phosphate | - |
Zea mays | D-glucose 6-phosphate | - |
? | |
| alpha-D-mannose 1-phosphate | - |
Zea mays | alpha-D-mannose 6-phosphate | - |
r | |
| D-Mannose 1-phosphate | - |
Zea mays | D-Mannose 6-phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 66000, SDS-PAGE | Zea mays |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PGM | - |
Zea mays |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
no loss of activity after 3 min | Zea mays |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Zea mays |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.23 | - |
D-Glucose 1,6-bisphosphate | 25°C, pH 7.6, substrate inhibition at high concentrations | Zea mays | |
| 1.35 | - |
D-mannose 1-phosphate | 25°C, pH 7.6, substrate inhibition at high concentrations | Zea mays | |
| 3.3 | - |
D-glucose 1-phosphate | 25°C, pH 7.6, substrate inhibition at high concentrations | Zea mays | |
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