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Literature summary for 5.4.2.2 extracted from
- Purification and characterization of two phosphoglucomutases from Lactococcus lactis subsp. lactis and their regulation in maltose- and glucose-utilizing cells (1994), J. Bacteriol., 176, 5304-5311.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| alpha-D-glucose 1,6-diphosphate | required | Lactococcus lactis |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Lactococcus lactis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 65000 | - |
gel filtration, SDS-PAGE, zymogram analysis | Lactococcus lactis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-D-glucose 1-phosphate | Lactococcus lactis | degradation of lactose in lactic acid bacteria | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lactococcus lactis | - |
subsp. lactis | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Lactococcus lactis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Lactococcus lactis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-D-glucose 1-phosphate | - |
Lactococcus lactis | alpha-D-glucose 6-phosphate | - |
? | |
| alpha-D-glucose 1-phosphate | degradation of lactose in lactic acid bacteria | Lactococcus lactis | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 65000, SDS-PAGE | Lactococcus lactis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | - |
12 h, 30% loss of activity | Lactococcus lactis |
| 50 | - |
15 min, 97% loss of activity | Lactococcus lactis |
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