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Literature summary for 5.4.2.12 extracted from

  • la Cruz, A.; Sanabria-Chanaga, E.; Yepez-Mulia, L.; Castillo, R.; Hernandez-Campos, A.; Najera, H.; Avitia-Dominguez, C.; Sierra-Campos, E.; Valdez-Solana, M.; Tellez-Valencia, A.
    Structural characterization, biochemical, inhibition and computational studies of Entamoeba histolytica phosphoglycerate mutase finding hits for a new antiamoebic drug (2018), Med. Chem. Res., 27, 1705-1716 .
No PubMed abstract available
Search for more literature for 5.4.2.12

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BLR(DE3) cells Entamoeba histolytica

Inhibitors

Inhibitors Comment Organism Structure
1-methyl-2-(methylthio)-1H-benzimidazole-5-carboxamide 54% inhibition at 0.1 mM Entamoeba histolytica
1-methyl-2-(methylthio)-N-(5-nitro-1,3-thiazol-2-yl)-1H-benzimidazole-6-carboxamide 47% inhibition at 0.1 mM Entamoeba histolytica
1H-benzimidazole-5-carboxylic acid 69% inhibition at 0.1 mM Entamoeba histolytica
2-(4-nitrophenyl)-1H-benzimidazole-7-carboxylic acid 56% inhibition at 0.1 mM Entamoeba histolytica
6-chloro-2-(4-nitrophenyl)-1H-benzimidazole-7-carboxylic acid 59% inhibition at 0.1 mM Entamoeba histolytica
6-chloro-2-(methylthio)-1H-benzimidazole-5-carboxylic acid 73% inhibition at 0.1 mM Entamoeba histolytica
6-chloro-2-(methylthio)-N-(5-nitro-1,3-thiazol-2-yl)-1H-benzimidazole-5-carboxamide 47% inhibition at 0.1 mM Entamoeba histolytica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.773
-
 3-phospho-D-glycerate at pH 6.0 and 37°C Entamoeba histolytica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57000
-
 gel filtration Entamoeba histolytica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-phospho-D-glycerate Entamoeba histolytica
-
 3-phospho-D-glycerate
-
 r
2-phospho-D-glycerate Entamoeba histolytica HM1 IMSS
-
 3-phospho-D-glycerate
-
 r
3-phospho-D-glycerate Entamoeba histolytica
-
 2-phospho-D-glycerate
-
 r
3-phospho-D-glycerate Entamoeba histolytica HM1 IMSS
-
 2-phospho-D-glycerate
-
 r

Organism

Organism UniProt Comment Textmining
Entamoeba histolytica C4LTW4
-
-
Entamoeba histolytica HM1 IMSS C4LTW4
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Entamoeba histolytica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-phospho-D-glycerate
-
 Entamoeba histolytica 3-phospho-D-glycerate
-
 r
2-phospho-D-glycerate
-
 Entamoeba histolytica HM1 IMSS 3-phospho-D-glycerate
-
 r
3-phospho-D-glycerate
-
 Entamoeba histolytica 2-phospho-D-glycerate
-
 r
3-phospho-D-glycerate
-
 Entamoeba histolytica HM1 IMSS 2-phospho-D-glycerate
-
 r

Subunits

Subunits Comment Organism
monomer 1 * 64000, SDS-PAGE Entamoeba histolytica

Synonyms

Synonyms Comment Organism
PGAM
-
 Entamoeba histolytica

Cofactor

Cofactor Comment Organism Structure
additional information the enzyme is independent from 2,3-bisphosphoglycerate Entamoeba histolytica