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Literature summary for 5.4.2.12 extracted from

  • Roychowdhury, A.; Kundu, A.; Gujar, A.; Bose, M.; Das, A.K.
    Expression, purification, crystallization and preliminary X-ray diffraction studies of phosphoglycerate mutase from Staphylococcus aureus NCTC8325 (2014), Acta Crystallogr. Sect. F, 70, 53-56 .
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli M15 (pREP4) cells Staphylococcus aureus

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, using either 0.1 M HEPES pH 7.5, 20% (w/v) polyethylene glycol 10000 or 0.2 M NaCl, 0.1 M bis-Tris pH 6.5, 25% (w/v) polyethylene glycol 3350, at 25°C Staphylococcus aureus

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ dependent on Staphylococcus aureus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-phospho-D-glycerate Staphylococcus aureus
-
 3-phospho-D-glycerate
-
 ?
2-phospho-D-glycerate Staphylococcus aureus NCTC 8325
-
 3-phospho-D-glycerate
-
 ?

Organism

Organism UniProt Comment Textmining
Staphylococcus aureus Q2G029
-
-
Staphylococcus aureus NCTC 8325 Q2G029
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA Sepharose column chromatography and Superdex 200 gel filtration Staphylococcus aureus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-phospho-D-glycerate
-
 Staphylococcus aureus 3-phospho-D-glycerate
-
 ?
2-phospho-D-glycerate
-
 Staphylococcus aureus NCTC 8325 3-phospho-D-glycerate
-
 ?

Synonyms

Synonyms Comment Organism
iPGM
-
 Staphylococcus aureus

Cofactor

Cofactor Comment Organism Structure
additional information the enzyme is not dependent from 2,3-bisphosphoglycerate Staphylococcus aureus