Literature summary for 5.4.2.12 extracted from

Nowicki, M.W.; Kuaprasert, B.; McNae, I.W.; Morgan, H.P.; Harding, M.M.; Michels, P.A.; Fothergill-Gilmore, L.A.; Walkinshaw, M.D.
Crystal structures of Leishmania mexicana phosphoglycerate mutase suggest a one-metal mechanism and a new enzyme subclass (2009), J. Mol. Biol., 394, 535-543.

Search for more literature for 5.4.2.12

Cloned(Commentary)

Cloned (Comment)	Organism
His-tagged enzyme is expressed in Escherichia coli BL21 cells	Leishmania mexicana

Crystallization (Commentary)

Crystallization (Comment)	Organism
iPGAM crystallized with 3-phosphoglycerate or 2-phosphoglycerate at high and low cobalt concentrations (4 mM and 0.01 mM), hanging drop vapor diffusion method, using 0.08 M ammonium acetate, 0.04 M trisodium citrate dihydrate pH 6.0, and 24% (w/v) PEG 4000	Leishmania mexicana

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Co2+	two Co2+ are coordinated within the active site	Leishmania mexicana

Organism

Organism	UniProt	Comment	Textmining
Leishmania mexicana	Q86N96	-	-

Purification (Commentary)

Purification (Comment)	Organism
metal affinity Talon column chromatography	Leishmania mexicana

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-phospho-D-glycerate	-	Leishmania mexicana	3-phospho-D-glycerate	-	r

Synonyms

Synonyms	Comment	Organism
cofactor-independent phosphoglycerate mutase 1	-	Leishmania mexicana
iPGAM	-	Leishmania mexicana

Cofactor

Cofactor	Comment	Organism	Structure
additional information	phosphoglycerate mutase is cofactor-independent	Leishmania mexicana

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Release 2023.1 (January 2023)