Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2,3-bisphosphoglycerate | strictly required, Km value 0.017 mM | Archaeoglobus fulgidus | |
2,3-bisphosphoglycerate | strictly required, Km value 0.017 mM | Thermoplasma acidophilum |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Thermoplasma acidophilum |
expression in Escherichia coli with His-tag | Archaeoglobus fulgidus |
Protein Variants | Comment | Organism |
---|---|---|
H23A | no enzymic activity. Like wild-type, mutant forms a dimer | Thermoplasma acidophilum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
vanadate | up to 0.1 mM, 90% maximal inhibition. Inhibition may be partially reversed by EDTA | Thermoplasma acidophilum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
2-phospho-D-glycerate | pH 7.0, 50°C | Archaeoglobus fulgidus | |
1.1 | - |
2-phospho-D-glycerate | pH 7.0, 50°C | Thermoplasma acidophilum | |
3.3 | - |
3-phospho-D-glycerate | pH 7.0, 50°C | Archaeoglobus fulgidus | |
3.3 | - |
3-phospho-D-glycerate | pH 7.0, 50°C | Thermoplasma acidophilum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | Mn2+ and Mg2+ are required for thermal stability | Archaeoglobus fulgidus | |
Mn2+ | in presence of 5 mM Mg2+, 2-3fold stimulation by 0.05 mM Mn2+. Mn2+ and Mg2+ are required for thermal stability | Archaeoglobus fulgidus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23800 | - |
2 * 23800, calculated, 2 * 24000, SDS-PAGE | Thermoplasma acidophilum |
24000 | - |
2 * 23800, calculated, 2 * 24000, SDS-PAGE | Thermoplasma acidophilum |
44100 | - |
1 * 44100, calculated, 1 * 48000, SDS-PAGE of His-tagged protein | Archaeoglobus fulgidus |
46000 | - |
gel filtration | Archaeoglobus fulgidus |
46000 | - |
gel filtration | Thermoplasma acidophilum |
48000 | - |
1 * 44100, calculated, 1 * 48000, SDS-PAGE of His-tagged protein | Archaeoglobus fulgidus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Archaeoglobus fulgidus | - |
cofactor-independent isoform | - |
Thermoplasma acidophilum | Q9HL27 | cofactor-dependent isoform | - |
Renatured (Comment) | Organism |
---|---|
inhibition of enzyme by vanadate may be partially reversed by EDTA | Thermoplasma acidophilum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.3 | - |
pH 7.0, 50°C | Archaeoglobus fulgidus |
12 | - |
pH 7.0, 50°C | Thermoplasma acidophilum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-phospho-D-glycerate | - |
Archaeoglobus fulgidus | 3-phospho-D-glycerate | - |
r | |
2-phospho-D-glycerate | - |
Thermoplasma acidophilum | 3-phospho-D-glycerate | - |
r | |
3-phospho-D-glycerate | - |
Archaeoglobus fulgidus | 2-phospho-D-glycerate | - |
r | |
3-phospho-D-glycerate | - |
Thermoplasma acidophilum | 2-phospho-D-glycerate | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 23800, calculated, 2 * 24000, SDS-PAGE | Thermoplasma acidophilum |
monomer | 1 * 44100, calculated, 1 * 48000, SDS-PAGE of His-tagged protein | Archaeoglobus fulgidus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
120 min, no loss of activity | Archaeoglobus fulgidus |
70 | - |
half-life 150 min in presence of Mn2+ and Mg2+. Half-life 30 min in the absence of Mn2+ and Mg2+ | Archaeoglobus fulgidus |
85 | - |
half-life 15 min in presence of Mn2+ and Mg2+ | Archaeoglobus fulgidus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.1 | - |
- |
Archaeoglobus fulgidus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | - |
about 40% of maximal activity | Archaeoglobus fulgidus |
7.7 | - |
about 70% of maximal activity | Archaeoglobus fulgidus |