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Literature summary for 5.4.2.12 extracted from

  • Johnsen, U.; Schoenheit, P.
    Characterization of cofactor-dependent and cofactor-independent phosphoglycerate mutases from Archaea (2007), Extremophiles, 11, 647-657.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
2,3-bisphosphoglycerate strictly required, Km value 0.017 mM Archaeoglobus fulgidus
2,3-bisphosphoglycerate strictly required, Km value 0.017 mM Thermoplasma acidophilum

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Thermoplasma acidophilum
expression in Escherichia coli with His-tag Archaeoglobus fulgidus

Protein Variants

Protein Variants Comment Organism
H23A no enzymic activity. Like wild-type, mutant forms a dimer Thermoplasma acidophilum

Inhibitors

Inhibitors Comment Organism Structure
vanadate up to 0.1 mM, 90% maximal inhibition. Inhibition may be partially reversed by EDTA Thermoplasma acidophilum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
2-phospho-D-glycerate pH 7.0, 50°C Archaeoglobus fulgidus
1.1
-
2-phospho-D-glycerate pH 7.0, 50°C Thermoplasma acidophilum
3.3
-
3-phospho-D-glycerate pH 7.0, 50°C Archaeoglobus fulgidus
3.3
-
3-phospho-D-glycerate pH 7.0, 50°C Thermoplasma acidophilum

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ Mn2+ and Mg2+ are required for thermal stability Archaeoglobus fulgidus
Mn2+ in presence of 5 mM Mg2+, 2-3fold stimulation by 0.05 mM Mn2+. Mn2+ and Mg2+ are required for thermal stability Archaeoglobus fulgidus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23800
-
2 * 23800, calculated, 2 * 24000, SDS-PAGE Thermoplasma acidophilum
24000
-
2 * 23800, calculated, 2 * 24000, SDS-PAGE Thermoplasma acidophilum
44100
-
1 * 44100, calculated, 1 * 48000, SDS-PAGE of His-tagged protein Archaeoglobus fulgidus
46000
-
gel filtration Archaeoglobus fulgidus
46000
-
gel filtration Thermoplasma acidophilum
48000
-
1 * 44100, calculated, 1 * 48000, SDS-PAGE of His-tagged protein Archaeoglobus fulgidus

Organism

Organism UniProt Comment Textmining
Archaeoglobus fulgidus
-
cofactor-independent isoform
-
Thermoplasma acidophilum Q9HL27 cofactor-dependent isoform
-

Renatured (Commentary)

Renatured (Comment) Organism
inhibition of enzyme by vanadate may be partially reversed by EDTA Thermoplasma acidophilum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.3
-
pH 7.0, 50°C Archaeoglobus fulgidus
12
-
pH 7.0, 50°C Thermoplasma acidophilum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-phospho-D-glycerate
-
Archaeoglobus fulgidus 3-phospho-D-glycerate
-
r
2-phospho-D-glycerate
-
Thermoplasma acidophilum 3-phospho-D-glycerate
-
r
3-phospho-D-glycerate
-
Archaeoglobus fulgidus 2-phospho-D-glycerate
-
r
3-phospho-D-glycerate
-
Thermoplasma acidophilum 2-phospho-D-glycerate
-
r

Subunits

Subunits Comment Organism
dimer 2 * 23800, calculated, 2 * 24000, SDS-PAGE Thermoplasma acidophilum
monomer 1 * 44100, calculated, 1 * 48000, SDS-PAGE of His-tagged protein Archaeoglobus fulgidus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
120 min, no loss of activity Archaeoglobus fulgidus
70
-
half-life 150 min in presence of Mn2+ and Mg2+. Half-life 30 min in the absence of Mn2+ and Mg2+ Archaeoglobus fulgidus
85
-
half-life 15 min in presence of Mn2+ and Mg2+ Archaeoglobus fulgidus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.1
-
-
Archaeoglobus fulgidus

pH Range

pH Minimum pH Maximum Comment Organism
6
-
about 40% of maximal activity Archaeoglobus fulgidus
7.7
-
about 70% of maximal activity Archaeoglobus fulgidus