Literature summary for 5.4.2.12 extracted from

Poonperm, B.; Guerra, D.G.; McNae, I.W.; Fothergill-Gilmore, L.A.; Walkinshaw, M.D.
Expression, purification, crystallization and preliminary crystallographic analysis of Leishmania mexicana phosphoglycerate mutase (2003), Acta Crystallogr. Sect. D, 59, 1313-1316.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Leishmania mexicana

Crystallization (Commentary)

Crystallization (Comment)	Organism
crystals of iPGAM complexed with 3-phosphoglycerate and Co2+, hanging-drop vapor diffusion, crystallization drops contain equal volumes of reservoir and enzyme solution, 5 mg/ml iPGAM, 1.5 mM 3-phosphoglycerate, 50 mM NaCl, 0.01 mM CoCl2 in 20 mM TEA buffer, pH 7.4, crystals diffract to 1.9 A	Leishmania mexicana

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
2-phospho-D-glycerate	Leishmania mexicana	-	3-phospho-D-glycerate	-	r

Organism

Organism	UniProt	Comment	Textmining
Leishmania mexicana	Q86N96	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant iPGAM	Leishmania mexicana

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-phospho-D-glycerate	-	Leishmania mexicana	3-phospho-D-glycerate	-	r

Synonyms

Synonyms	Comment	Organism
iPGAM	-	Leishmania mexicana

Cofactor

Cofactor	Comment	Organism	Structure
additional information	2,3-bisphosphoglycerate-independent iPGAM	Leishmania mexicana

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Release 2023.1 (January 2023)