Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | no influence on activity: fructose 6-phosphate, fructose 1,6-bisphosphate, glucose 6-phosphate, citric acid, malate, glyoxylate, phosphoenolpyruvate, pyruvate, phosphoglycolate, glyceric acid, alpha-glycerophosphate, iodoacetamide, Gly, Ser, diphosphate, 2,3-diglycerophosphate | Ricinus communis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | no influence on activity: fructose 6-phosphate, fructose 1,6-bisphosphate, glucose 6-phosphate, citric acid, malate, glyoxylate, phosphoenolpyruvate, pyruvate, phosphoglycolate, glyceric acid, alpha-glycerophosphate, iodoacetamide, Gly, Ser, diphosphate, 2,3-diglycerophosphate | Ricinus communis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
2-phospho-D-glycerate | 2-phosphoglycerate, cytosolic enzyme | Ricinus communis | |
0.112 | - |
2-phospho-D-glycerate | plastidic enzyme | Ricinus communis | |
0.33 | - |
3-phospho-D-glycerate | cytosolic enzyme | Ricinus communis | |
0.43 | - |
3-phospho-D-glycerate | plastidic enzyme | Ricinus communis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Ricinus communis | 5829 | - |
plastid | - |
Ricinus communis | 9536 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no requirement for divalent metal ions | Ricinus communis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
63500 | - |
plastidic enzyme, gel filtration | Ricinus communis |
64500 | - |
cytosolic enzyme, gel filtration | Ricinus communis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ricinus communis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Ricinus communis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seed | - |
Ricinus communis | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
83.8 | - |
plastidic enzyme | Ricinus communis |
622.5 | - |
cytosolic enzyme | Ricinus communis |
Storage Stability | Organism |
---|---|
-20°C, potassium phosphate buffer, pH 7.2, 20-25% glycerol, 1 month, no loss of activity | Ricinus communis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-phospho-D-glycerate | - |
Ricinus communis | 3-phospho-D-glycerate | - |
r | |
3-phospho-D-glycerate | - |
Ricinus communis | 2-phospho-D-glycerate | - |
r |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
plastidic enzyme: 50 min, 50% loss of activity, cytosolic enzyme: 125 min, 50% loss of activity, no protection by 2-phosphoglycerate, 3-phosphoglycerate, 2,3-bisphosphoglycerate, phosphate | Ricinus communis |
60 | - |
plastidic enzyme: 8 min, 50% loss of activity, cytosolic enzyme: 25 min, 50% loss of activity, no protection by 2-phosphoglycerate, 3-phosphoglycerate, 2,3-bisphosphoglycerate, phosphate | Ricinus communis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
cytosolic enzyme, substrate 3-phosphoglycerate or 2-phosphoglycerate | Ricinus communis |
7.3 | - |
plastidic enzyme, substrate 2-phosphoglycerate | Ricinus communis |
7.5 | - |
plastidic enzyme, substrate 3-phoshoglycerate | Ricinus communis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
8 h, 4°C, complete inactivation | Ricinus communis |
6.8 | - |
rapid inactivation | Ricinus communis |
11 | - |
24 h, 4°C, 15% loss of activity | Ricinus communis |