Literature summary for 5.4.2.11 extracted from

Nairn, J.; Duncan, D.; Price, N.E.; Kelly, S.M.; Fothergill-Gilmore, L.A.; Uhrinova, S.; Barlow, P.N.; Rigden, D.J.; Price, N.C.
Characterization of active-site mutants of Schizosaccharomyces pombe phosphoglycerate mutase. Elucidation of the roles of amino acids involved in substrate binding and catalysis (2000), Eur. J. Biochem., 267, 7065-7074.

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Activating Compound

Activating Compound	Comment	Organism	Structure
2,3-bisphosphoglycerate	-	Schizosaccharomyces pombe
2-phosphoglycollate	slight activation of phosphatase activity	Schizosaccharomyces pombe

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Saccharomyces cerevisiae	Schizosaccharomyces pombe

Protein Variants

Protein Variants	Comment	Organism
E93D	less than 1% of wild-type activity	Schizosaccharomyces pombe
R120Q	59% of wild-type activity	Schizosaccharomyces pombe
R120Q/R121Q	62% of wild-type activity	Schizosaccharomyces pombe
R121Q	12% of wild-type activity	Schizosaccharomyces pombe
R14Q	less than 1% of wild-type activity	Schizosaccharomyces pombe
R94Q	1% of wild-type activity	Schizosaccharomyces pombe
S62A	loss of activity during purification	Schizosaccharomyces pombe

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.58	-	3-phosphoglycerate	-	Schizosaccharomyces pombe
1.25	-	3-phosphoglycerate	E93D mutant	Schizosaccharomyces pombe
1.54	-	3-phosphoglycerate	R14Q mutant	Schizosaccharomyces pombe
2.64	-	3-phosphoglycerate	R120Q mutant	Schizosaccharomyces pombe
4.9	-	3-phosphoglycerate	R121Q mutant	Schizosaccharomyces pombe
5.26	-	3-phosphoglycerate	R120Q/R121Q double mutant	Schizosaccharomyces pombe
7.46	-	3-phosphoglycerate	R94Q mutant	Schizosaccharomyces pombe

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
2-phospho-D-glycerate	Schizosaccharomyces pombe	-	3-phospho-D-glycerate	-	r

Organism

Organism	UniProt	Comment	Textmining
Schizosaccharomyces pombe	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-phospho-D-glycerate	-	Schizosaccharomyces pombe	3-phospho-D-glycerate	-	r
3-Phosphoglycerate	-	Schizosaccharomyces pombe	2-Phosphoglycerate	-	?

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Release 2023.1 (January 2023)