Activating Compound | Comment | Organism | Structure |
---|---|---|---|
D-glucosamine 1,6-diphosphate | retains the enzyme in an active and phosphorylated form | Escherichia coli | |
D-glucose 1,6-diphosphate | retains the enzyme in an active and phosphorylated form | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
S100A | very low activity | Escherichia coli |
S100T | contributes to the specificity towards sugar- or aminosugar-phosphates, much more efficient in catalysis of the phosphoglucose mutase reaction | Escherichia coli |
S102A | site of phosphorylation, no activity | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
D-glucosamine 6-phosphate | - |
Escherichia coli | |
0.08 | - |
D-glucosamine 1-phosphate | - |
Escherichia coli | |
0.08 | - |
D-glucosamine 1,6-diphosphate | - |
Escherichia coli | |
0.08 | - |
D-glucose 1-phosphate | single mutation S100T | Escherichia coli | |
0.5 | - |
D-Glucose 1,6-bisphosphate | - |
Escherichia coli | |
0.65 | - |
D-glucose 1-phosphate | - |
Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47412 | - |
3 * 47412, gel filtration, mass spectrometry | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucoamine 6-phosphate | Escherichia coli | ping-pong bi-bi reaction mechanism | D-glucosamine 1-phosphate | - |
r | |
D-glucoamine 6-phosphate | Escherichia coli | two basically different reaction sequences, 1. phosphate group transfer yielding the intermediate D-GlcN-1,6-diphosphate, 2. conversion to GlcN-1-phosphate | D-glucosamine 1-phosphate | - |
r | |
D-glucoamine 6-phosphate | Escherichia coli | involved in formation of cell-wall precursor UDP-N-acetylglucosamine | D-glucosamine 1-phosphate | - |
r | |
D-glucosamine 1-phosphate | Escherichia coli | - |
D-glucosamine 6-phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | the only phosphogroup at Ser102 | Escherichia coli |
Purification (Comment) | Organism |
---|---|
near homogeneity | Escherichia coli |
near homogeneity, recombinant enzyme | Escherichia coli |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Escherichia coli | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.007 | - |
substrate: D-glucose 6-phosphate | Escherichia coli |
3 | - |
6x His-tagged recombinant enzyme | Escherichia coli |
10 | - |
substrate: D-glucosamine 6-phosphate | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucoamine 6-phosphate | ping-pong bi-bi reaction mechanism | Escherichia coli | D-glucosamine 1-phosphate | - |
r | |
D-glucoamine 6-phosphate | two basically different reaction sequences, 1. phosphate group transfer yielding the intermediate D-GlcN-1,6-diphosphate, 2. conversion to GlcN-1-phosphate | Escherichia coli | D-glucosamine 1-phosphate | - |
r | |
D-glucoamine 6-phosphate | involved in formation of cell-wall precursor UDP-N-acetylglucosamine | Escherichia coli | D-glucosamine 1-phosphate | - |
r | |
D-glucosamine 1,6-diphosphate | - |
Escherichia coli | ? | - |
? | |
D-glucosamine 1-phosphate | - |
Escherichia coli | D-glucosamine 6-phosphate | - |
r | |
D-glucose 1,6-bisphosphate | - |
Escherichia coli | ? | - |
? | |
D-glucose 1-phosphate | in the presence of Glc-1,6-diphosphate, 1400fold lower activity than for D-glucosamine 1-phosphate or D-glucosamine 1,6-diphosphate, ping-pong reaction mechanism | Escherichia coli | D-glucose 6-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
trimer | 3 * 47412, gel filtration, mass spectrometry | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0055 | - |
D-glucose 1-phosphate | - |
Escherichia coli | |
0.0138 | - |
D-glucose 1-phosphate | single-mutation S100T | Escherichia coli | |
2.42 | - |
D-glucosamine 6-phosphate | 6 x His-tagged recombinant enzyme | Escherichia coli | |
7.9 | - |
D-glucosamine 6-phosphate | - |
Escherichia coli |