BRENDA - Enzyme Database
show all sequences of 5.4.2.1

Identification and characterization of the cofactor-independent phosphoglycerate mutases of Dirofilaria immitis and its Wolbachia endosymbiont

Li, Z.; Galvin, B.D.; Raverdy, S.; Carlow, C.K.; Vet. Parasitol. 176, 350-356 (2011)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli C2566/T7 cells
Dirofilaria immitis
expressed in Escherichia coli C2566/T7 cells
Wolbachia endosymbiont of Dirofilaria immitis
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.55
-
3-phospho-D-glycerate
in 30 mM Tris-HCl, 5 mM MgSO4, 20 mM KCl, pH 7.5, at 30°C
Wolbachia endosymbiont of Dirofilaria immitis
0.58
-
3-phospho-D-glycerate
in 30 mM Tris-HCl, 5 mM MgSO4, 20 mM KCl, pH 7.5, at 30°C
Dirofilaria immitis
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
56000
-
x * 56000, calculated from amino acid sequence
Wolbachia endosymbiont of Dirofilaria immitis
57000
-
x * 57000, calculated from amino acid sequence
Dirofilaria immitis
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Dirofilaria immitis
F4ZE01
-
-
Wolbachia endosymbiont of Dirofilaria immitis
F4ZE02
-
-
Purification (Commentary)
Commentary
Organism
HiTrap nickel chelating column chromatography and HiTrap Q column chromatography
Dirofilaria immitis
HiTrap nickel chelating column chromatography and HiTrap Q column chromatography
Wolbachia endosymbiont of Dirofilaria immitis
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
71
-
in 30 mM Tris-HCl, 5 mM MgSO4, 20 mM KCl, pH 7.5, at 30°C
Wolbachia endosymbiont of Dirofilaria immitis
225
-
in 30 mM Tris-HCl, 5 mM MgSO4, 20 mM KCl, pH 7.5, at 30°C
Dirofilaria immitis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-phospho-D-glycerate
-
716984
Wolbachia endosymbiont of Dirofilaria immitis
3-phospho-D-glycerate
-
-
-
r
2-phospho-D-glycerate
-
716984
Dirofilaria immitis
3-phospho-D-glycerate
-
-
-
r
3-phospho-D-glycerate
-
716984
Wolbachia endosymbiont of Dirofilaria immitis
2-phospho-D-glycerate
-
-
-
r
3-phospho-D-glycerate
-
716984
Dirofilaria immitis
2-phospho-D-glycerate
-
-
-
r
Subunits
Subunits
Commentary
Organism
?
x * 57000, calculated from amino acid sequence
Dirofilaria immitis
?
x * 56000, calculated from amino acid sequence
Wolbachia endosymbiont of Dirofilaria immitis
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
102
-
3-phospho-D-glycerate
in 30 mM Tris-HCl, 5 mM MgSO4, 20 mM KCl, pH 7.5, at 30°C
Wolbachia endosymbiont of Dirofilaria immitis
132
-
3-phospho-D-glycerate
in 30 mM Tris-HCl, 5 mM MgSO4, 20 mM KCl, pH 7.5, at 30°C
Dirofilaria immitis
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
2,3-bisphosphoglycerate is not required as a cofactor
Dirofilaria immitis
additional information
2,3-bisphosphoglycerate is not required as a cofactor
Wolbachia endosymbiont of Dirofilaria immitis
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Dirofilaria immitis
calculated from amino acid sequence
-
6.3
Wolbachia endosymbiont of Dirofilaria immitis
calculated from amino acid sequence
-
7.4
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli C2566/T7 cells
Dirofilaria immitis
expressed in Escherichia coli C2566/T7 cells
Wolbachia endosymbiont of Dirofilaria immitis
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
2,3-bisphosphoglycerate is not required as a cofactor
Dirofilaria immitis
additional information
2,3-bisphosphoglycerate is not required as a cofactor
Wolbachia endosymbiont of Dirofilaria immitis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.55
-
3-phospho-D-glycerate
in 30 mM Tris-HCl, 5 mM MgSO4, 20 mM KCl, pH 7.5, at 30°C
Wolbachia endosymbiont of Dirofilaria immitis
0.58
-
3-phospho-D-glycerate
in 30 mM Tris-HCl, 5 mM MgSO4, 20 mM KCl, pH 7.5, at 30°C
Dirofilaria immitis
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
56000
-
x * 56000, calculated from amino acid sequence
Wolbachia endosymbiont of Dirofilaria immitis
57000
-
x * 57000, calculated from amino acid sequence
Dirofilaria immitis
Purification (Commentary) (protein specific)
Commentary
Organism
HiTrap nickel chelating column chromatography and HiTrap Q column chromatography
Dirofilaria immitis
HiTrap nickel chelating column chromatography and HiTrap Q column chromatography
Wolbachia endosymbiont of Dirofilaria immitis
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
71
-
in 30 mM Tris-HCl, 5 mM MgSO4, 20 mM KCl, pH 7.5, at 30°C
Wolbachia endosymbiont of Dirofilaria immitis
225
-
in 30 mM Tris-HCl, 5 mM MgSO4, 20 mM KCl, pH 7.5, at 30°C
Dirofilaria immitis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-phospho-D-glycerate
-
716984
Wolbachia endosymbiont of Dirofilaria immitis
3-phospho-D-glycerate
-
-
-
r
2-phospho-D-glycerate
-
716984
Dirofilaria immitis
3-phospho-D-glycerate
-
-
-
r
3-phospho-D-glycerate
-
716984
Wolbachia endosymbiont of Dirofilaria immitis
2-phospho-D-glycerate
-
-
-
r
3-phospho-D-glycerate
-
716984
Dirofilaria immitis
2-phospho-D-glycerate
-
-
-
r
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 57000, calculated from amino acid sequence
Dirofilaria immitis
?
x * 56000, calculated from amino acid sequence
Wolbachia endosymbiont of Dirofilaria immitis
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
102
-
3-phospho-D-glycerate
in 30 mM Tris-HCl, 5 mM MgSO4, 20 mM KCl, pH 7.5, at 30°C
Wolbachia endosymbiont of Dirofilaria immitis
132
-
3-phospho-D-glycerate
in 30 mM Tris-HCl, 5 mM MgSO4, 20 mM KCl, pH 7.5, at 30°C
Dirofilaria immitis
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Dirofilaria immitis
calculated from amino acid sequence
-
6.3
Wolbachia endosymbiont of Dirofilaria immitis
calculated from amino acid sequence
-
7.4
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
186
-
3-phospho-D-glycerate
in 30 mM Tris-HCl, 5 mM MgSO4, 20 mM KCl, pH 7.5, at 30°C
Wolbachia endosymbiont of Dirofilaria immitis
228
-
3-phospho-D-glycerate
in 30 mM Tris-HCl, 5 mM MgSO4, 20 mM KCl, pH 7.5, at 30°C
Dirofilaria immitis
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
186
-
3-phospho-D-glycerate
in 30 mM Tris-HCl, 5 mM MgSO4, 20 mM KCl, pH 7.5, at 30°C
Wolbachia endosymbiont of Dirofilaria immitis
228
-
3-phospho-D-glycerate
in 30 mM Tris-HCl, 5 mM MgSO4, 20 mM KCl, pH 7.5, at 30°C
Dirofilaria immitis
Other publictions for EC 5.4.2.1
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
716984
Li
Identification and characteriz ...
Dirofilaria immitis, Wolbachia endosymbiont of Dirofilaria immitis
Vet. Parasitol.
176
350-356
2011
-
-
2
-
-
-
-
2
-
-
2
-
-
5
-
-
2
-
-
-
2
-
4
2
-
-
-
2
-
-
-
2
-
2
-
-
-
2
2
-
-
-
-
-
-
2
-
-
2
-
-
-
-
2
-
-
2
-
4
2
-
-
-
2
-
-
-
2
-
-
-
-
2
2
715964
Solem
Phosphoglycerate mutase is a h ...
Lactococcus lactis subsp. lactis, Lactococcus lactis subsp. lactis IL1403
J. Mol. Microbiol. Biotechnol.
18
174-180
2010
1
-
-
-
-
-
1
1
-
-
2
-
-
4
-
-
-
-
-
-
-
-
2
1
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
1
-
-
2
-
-
-
-
-
-
-
-
-
2
1
-
-
-
1
-
-
-
-
-
1
1
-
-
-
727725
Yu
Pathway of glycogen metabolism ...
Methanococcus maripaludis
J. Bacteriol.
176
325-332
1994
3
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
1
-
-
-