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Literature summary for 5.4.1.4 extracted from

  • Bouvier, J.; Groninger-Poe, F.; Vetting, M.; Almo, S.; Gerlt, J.
    Galactaro delta-lactone isomerase Lactone isomerization by a member of the amidohydrolase superfamily (2014), Biochemistry, 53, 614-616 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.4.1.4

Protein Variants

Protein Variants Comment Organism
N240D the substitution produces a 28fold decrease in activity Agrobacterium tumefaciens

Metals/Ions

Metals/Ions Comment Organism Structure
additional information the presence of Ca2+, Co2+, Mg2+, Mn2+, Ni2+, or Zn2+ does not increase the rate of the enzymatic reaction Agrobacterium tumefaciens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-galactaro-1,5-lactone Agrobacterium tumefaciens
-
 D-galactaro-1,4-lactone
-
 ?
D-galactaro-1,5-lactone Agrobacterium tumefaciens C58
-
 D-galactaro-1,4-lactone
-
 ?

Organism

Organism UniProt Comment Textmining
Agrobacterium tumefaciens A9CEQ7
-
-
Agrobacterium tumefaciens C58 A9CEQ7
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-galactaro-1,5-lactone
-
 Agrobacterium tumefaciens D-galactaro-1,4-lactone
-
 ?
D-galactaro-1,5-lactone
-
 Agrobacterium tumefaciens C58 D-galactaro-1,4-lactone
-
 ?

Synonyms

Synonyms Comment Organism
D-galactarolactone isomerase
-
 Agrobacterium tumefaciens
galactaro delta-lactone isomerase
-
 Agrobacterium tumefaciens
GLI
-
 Agrobacterium tumefaciens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
16
-
 D-galactaro-1,5-lactone mutant enzyme N240D, pH and temperature not specified in the publication Agrobacterium tumefaciens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.6
-
 D-galactaro-1,5-lactone mutant enzyme N240D, pH and temperature not specified in the publication Agrobacterium tumefaciens