Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 5.3.99.7 extracted from

  • Oelschlaegel, M.; Groening, J.A.; Tischler, D.; Kaschabek, S.R.; Schloemann, M.
    Styrene oxide isomerase of Rhodococcus opacus 1CP, a highly stable and considerably active enzyme (2012), Appl. Environ. Microbiol., 78, 4330-4337.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.3.99.7

Inhibitors

Inhibitors Comment Organism Structure
phenylacetaldehyde irreversible product inhibition, half-life of 15 min determined at a phenylacetaldehyde concentration of about 55 mM Rhodococcus opacus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane integral membrane protein Rhodococcus opacus 16020
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
styrene oxide Rhodococcus opacus
-
 phenylacetaldehyde
-
 ?
styrene oxide Rhodococcus opacus 1CP
-
 phenylacetaldehyde
-
 ?

Organism

Organism UniProt Comment Textmining
Rhodococcus opacus
-
-
-
Rhodococcus opacus 1CP
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme partially from styrene-grown cells by membrane preparation and solubilization Rhodococcus opacus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.12
-
 styrene-grown cell extract, pH 7.2, 22°C Rhodococcus opacus
42.1
-
 partially purified enzyme, pH 7.2, 22°C Rhodococcus opacus
91.7
-
 partially purified enzyme, pH 7.2, 65°C Rhodococcus opacus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-styrene oxide 45% of activity compared to (S)-styrene oxide and (R,S)-styrene oxide Rhodococcus opacus phenylacetaldehyde
-
 ?
(R)-styrene oxide 45% of activity compared to (S)-styrene oxide and (R,S)-styrene oxide Rhodococcus opacus 1CP phenylacetaldehyde
-
 ?
(R,S)-3-chlorostyrene oxide
-
 Rhodococcus opacus 3-chlorophenylacetaldehyde
-
 ?
(R,S)-3-chlorostyrene oxide
-
 Rhodococcus opacus 1CP 3-chlorophenylacetaldehyde
-
 ?
(R,S)-4-bromostyrene oxide low activity Rhodococcus opacus 4-bromophenylacetaldehyde
-
 ?
(R,S)-4-bromostyrene oxide low activity Rhodococcus opacus 1CP 4-bromophenylacetaldehyde
-
 ?
(R,S)-4-chlorostyrene oxide low activity Rhodococcus opacus 4-chlorophenylacetaldehyde
-
 ?
(R,S)-4-fluorostyrene oxide
-
 Rhodococcus opacus 4-fluorophenylacetaldehyde
-
 ?
(R,S)-4-methylstyrene oxide
-
 Rhodococcus opacus 4-methylphenylacetaldehyde
-
 ?
(R,S)-styrene oxide best substrate Rhodococcus opacus phenylacetaldehyde
-
 ?
(S)-styrene oxide best substrate Rhodococcus opacus phenylacetaldehyde
-
 ?
additional information the enzyme shows the ability to convert a spectrum of substituted styrene oxides Rhodococcus opacus ?
-
 ?
additional information the enzyme shows the ability to convert a spectrum of substituted styrene oxides Rhodococcus opacus 1CP ?
-
 ?
styrene oxide
-
 Rhodococcus opacus phenylacetaldehyde
-
 ?
styrene oxide
-
 Rhodococcus opacus 1CP phenylacetaldehyde
-
 ?

Synonyms

Synonyms Comment Organism
SOOI
-
 Rhodococcus opacus
styrene oxide isomerase
-
 Rhodococcus opacus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
-
 Rhodococcus opacus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 70 activity range, wide temperature tolerance, profile overview Rhodococcus opacus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 40 partially purified native enzyme, 30 min, most stable at Rhodococcus opacus
65
-
 partially purified native enzyme, 30 min, inactivation Rhodococcus opacus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Rhodococcus opacus

pH Range

pH Minimum pH Maximum Comment Organism
additional information
-
 wide pH tolerance Rhodococcus opacus

pH Stability

pH Stability pH Stability Maximum Comment Organism
7
-
 partially purified native enzyme, 30 min, most stable at Rhodococcus opacus

Expression

Organism Comment Expression
Rhodococcus opacus the enzyme is strongly induced by styrene, to a lesser extent by styrene oxide and phenylacetaldehyde, no induction by phenylacetic acid or glucose up

General Information

General Information Comment Organism
metabolism the enzyme is part of the upper pathway of styrene degradation via side chain oxygenation Rhodococcus opacus