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Literature summary for 5.3.99.7 extracted from

  • Itoh, N.; Hayashi, K.; Okada, K.; Ito, T.; Mizuguchi, N.
    Characterization of Styrene Oxide Isomerase, a Key Enzyme of Styrene and Styrene Oxide Metabolism in Corynehacterium sp. (1997), Biosci. Biotechnol. Biochem., 61, 2058-2062.
    View publication on PubMed
Search for more literature for 5.3.99.7

Application

Application Comment Organism
synthesis the highly stereospecific enzyme can be used in a biotransformation system producing (R)-styrene oxide Corynebacterium sp.

Inhibitors

Inhibitors Comment Organism Structure
AgNO3 0.1 mM, 29% inhibition Corynebacterium sp.
dithiothreitol 1 mM, 22% inhibition Corynebacterium sp.
HgCl2 1 mM, 80% inhibition Corynebacterium sp.
hydrazine 1 mM, 82% inhibition Corynebacterium sp.
hydroxylammonium chloride 1 mM, 77% inhibition Corynebacterium sp.
iodoacetate 1 mM, 80% inhibition Corynebacterium sp.
KCN 1 mM, 32% inhibition Corynebacterium sp.
PCMB 1 mM, 24% inhibition Corynebacterium sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.077
-
 styrene oxide pH 7.0, 25°C Corynebacterium sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
additional information not membrane-bound, probably bound to the cell wall Corynebacterium sp.
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information non-metal enzyme Corynebacterium sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
styrene oxide + H2O Corynebacterium sp. key enzyme of styrene and styrene oxide metabolism phenylacetaldehyde
-
 ir
styrene oxide + H2O Corynebacterium sp. AC-5 key enzyme of styrene and styrene oxide metabolism phenylacetaldehyde
-
 ir

Organism

Organism UniProt Comment Textmining
Corynebacterium sp.
-
-
-
Corynebacterium sp. AC-5
-
-
-
Pseudomonas sp.
-
-
-
Pseudomonas sp. SK3
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Corynebacterium sp.

Source Tissue

Source Tissue Comment Organism Textmining
cell culture cells grown in a styrene gas atmosphere Corynebacterium sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
58.7
-
-
 Corynebacterium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
styrene oxide + H2O compared with the (R)-isomer, the relative activity of the enzyme towards (R)-styrene oxide is 51% of that for the (S)-isomer Corynebacterium sp. phenylacetaldehyde
-
 ?
styrene oxide + H2O key enzyme of styrene and styrene oxide metabolism Corynebacterium sp. phenylacetaldehyde
-
 ir
styrene oxide + H2O compared with the (R)-isomer, the relative activity of the enzyme towards (R)-styrene oxide is 51% of that for the (S)-isomer Corynebacterium sp. AC-5 phenylacetaldehyde
-
 ?
styrene oxide + H2O key enzyme of styrene and styrene oxide metabolism Corynebacterium sp. AC-5 phenylacetaldehyde
-
 ir

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Corynebacterium sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 pH 7.0, 1 h, stable Corynebacterium sp.
50
-
 pH 7.0, 1 h, 10% loss of activity Corynebacterium sp.
55
-
 pH 7.0, 1 h, 50% loss of activity Corynebacterium sp.
60
-
 pH 7.0, 1 h, 80% loss of activity Corynebacterium sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5 7.5
-
 Corynebacterium sp.

pH Range

pH Minimum pH Maximum Comment Organism
5 10 pH 5.0-7.5: maximal activity, pH 10.0: about 50% of maximal activity Corynebacterium sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
6
-
 25°C, 1 h, unstable below Corynebacterium sp.
7 8 25°C, 1 h Corynebacterium sp.
10
-
 25°C, 1 h, 10% loss of activity Corynebacterium sp.