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Literature summary for 5.3.99.6 extracted from

  • Schaller, F.; Zerbe, P.; Reinbothe, S.; Reinbothe, C.; Hofmann, E.; Pollmann, S.
    The allene oxide cyclase family of Arabidopsis thaliana: localization and cyclization (2008), FEBS J., 275, 2428-2441.
    View publication on PubMed
Search for more literature for 5.3.99.6

Protein Variants

Protein Variants Comment Organism
C71A 97.7% of wild-type activity, 94.1% yield of cis-(+)-12-oxophytodienoic acid Arabidopsis thaliana
C71S 97.1% of wild-type activity, 93.9% yield of cis-(+)-12-oxophytodienoic acid Arabidopsis thaliana
C71Y 33.3% of wild-type activity, 67.4% yield of cis-(+)-12-oxophytodienoic acid Arabidopsis thaliana
E23A complete loss of activity Arabidopsis thaliana
E23A 29% of wild-type activity, 57.6% yield of cis-(+)-12-oxophytodienoic acid Arabidopsis thaliana
F43Y 90.4% of wild-type activity, 93.5% yield of cis-(+)-12-oxophytodienoic acid Arabidopsis thaliana
F51A 128.4% of wild-type activity, 94.7% yield of cis-(+)-12-oxophytodienoic acid Arabidopsis thaliana
F85A 54.7% of wild-type activity, 71.7% yield of cis-(+)-12-oxophytodienoic acid Arabidopsis thaliana
F85L 79.6% of wild-type activity, 87.7% yield of cis-(+)-12-oxophytodienoic acid Arabidopsis thaliana
L27R 101.3% of wild-type activity, 92.8% yield of cis-(+)-12-oxophytodienoic acid Arabidopsis thaliana
N25L 38.6% of wild-type activity, 63.4% yield of cis-(+)-12-oxophytodienoic acid Arabidopsis thaliana
N53L 248% of wild-type activity, 83.4% yield of cis-(+)-12-oxophytodienoic acid Arabidopsis thaliana
P32V 61% of wild-type activity, 57.1% yield of cis-(+)-12-oxophytodienoic acid Arabidopsis thaliana
S31A 75.8% of wild-type activity, 71.8% yield of cis-(+)-12-oxophytodienoic acid Arabidopsis thaliana
S31A/N53L 93.6% of wild-type activity, 84.9% yield of cis-(+)-12-oxophytodienoic acid Arabidopsis thaliana
V49F 130.4% of wild-type activity, 93.0% yield of cis-(+)-12-oxophytodienoic acid Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
 Arabidopsis thaliana 9507
-

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q93ZC5 isoforms AOC4
-
Arabidopsis thaliana Q9LS01 isoform AOC3
-
Arabidopsis thaliana Q9LS02 isoforms AOC2
-
Arabidopsis thaliana Q9LS03 isoforms AOC1
-

Reaction

Reaction Comment Organism Reaction ID
(9Z)-(13S)-12,13-epoxyoctadeca-9,11,15-trienoate = (15Z)-12-oxophyto-10,15-dienoate the transition state with a pentadienyl carbocation and an oxyanion is stabilized by a strongly bound water molecule and favorable pi-pi interactions with aromatic residues in the cavity. Stereoselectivity results from steric restrictions to the necessary substrate isomerizations imposed by the protein environment Arabidopsis thaliana
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
13(S)-hydroperoxy-9(Z),11(E),15(Z)-octadecatrienoic acid
-
 Arabidopsis thaliana cis-(+)-12-oxophytodienoic acid 93.9% of cis-(+)-12-oxophytodienoic acid ?
13(S)-hydroperoxy-9(Z),11(E),15(Z)-octadecatrienoic acid
-
 Arabidopsis thaliana cis-(+)-12-oxophytodienoic acid 94.1% of cis-(+)-12-oxophytodienoic acid ?
13(S)-hydroperoxy-9(Z),11(E),15(Z)-octadecatrienoic acid
-
 Arabidopsis thaliana cis-(+)-12-oxophytodienoic acid isoform AOC1, 91.3% of cis-(+)-12-oxophytodienoic acid ?
13(S)-hydroperoxy-9(Z),11(E),15(Z)-octadecatrienoic acid
-
 Arabidopsis thaliana cis-(+)-12-oxophytodienoic acid isoforms AOC2, 93.6%of cis-(+)-12-oxophytodienoic acid ?
additional information all Arabidopsis allene oxide cyclase isoforms may contribute to jasmonate biosynthesis Arabidopsis thaliana ?
-
 ?