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Literature summary for 5.3.99.3 extracted from

  • Hamza, A.; Tong, M.; AbdulHameed, M.D.; Liu, J.; Goren, A.C.; Tai, H.H.; Zhan, C.G.
    Understanding microscopic binding of human microsomal prostaglandin E synthase-1 (mPGES-1) trimer with substrate PGH2 and cofactor GSH: insights from computational alanine scanning and site-directed mutagenesis (2010), J. Phys. Chem. B, 114, 5605-5616.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in 293-F cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
H72A the mutation markedly decreases the mPGES-1 activity (32%) Homo sapiens
N74A the mutant shows increased Km compared to the wild type enzyme Homo sapiens
R122A the mutant shows increased Km compared to the wild type enzyme Homo sapiens
R70A the mutant shows increased Km compared to the wild type enzyme Homo sapiens
R70A/R122A the mutant shows increased Km compared to the wild type enzyme, the double mutant of the mPGES-1 significantly reduces the binding affinity of GSH Homo sapiens
T129V the mutant shows increased Km compared to the wild type enzyme Homo sapiens
Y117A the mutant shows increased Km compared to the wild type enzyme Homo sapiens
Y117F the mutant still maintains full catalytic activity relative to the wild type Homo sapiens
Y117S the mutant shows increased Km compared to the wild type enzyme Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.215
-
prostaglandin H2 wild type enzyme Homo sapiens
0.2832
-
prostaglandin H2 mutant enzyme Y117S Homo sapiens
0.296
-
prostaglandin H2 mutant enzyme R70A Homo sapiens
0.372
-
prostaglandin H2 mutant enzyme R122A Homo sapiens
0.3842
-
prostaglandin H2 mutant enzyme T129V Homo sapiens
0.4643
-
prostaglandin H2 mutant enzyme N74A Homo sapiens
0.4737
-
prostaglandin H2 mutant enzyme R70A/R122A Homo sapiens
0.5345
-
prostaglandin H2 mutant enzyme Y117A Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
Homo sapiens
-
-

Organism

Organism UniProt Comment Textmining
Homo sapiens O14684
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Prostaglandin H2
-
Homo sapiens Prostaglandin E2
-
?

Subunits

Subunits Comment Organism
trimer
-
Homo sapiens

Synonyms

Synonyms Comment Organism
microsomal prostaglandin E synthase-1
-
Homo sapiens
mPGES-1
-
Homo sapiens

Cofactor

Cofactor Comment Organism Structure
glutathione
-
Homo sapiens