Literature summary for 5.3.99.3 extracted from

Kobayashi, T.; Nakatani, Y.; Tanioka, T.; Tsujimoto, M.; Nakajo, S.; Nakaya, K.; Murakami, M.; Kudo, I.
Regulation of cytosolic prostaglandin E synthase by phosphorylation (2004), Biochem. J., 381, 59-69.

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Activating Compound

Activating Compound	Comment	Organism	Structure
A23187	-	Rattus norvegicus
bradykinin	-	Rattus norvegicus
Hsp90	maximal activity is achieved when the three components (cPGES, casein kinase II and Hsp90) are mixed at a molar proportion of 1:1:1	Rattus norvegicus
additional information	phosphorylation is crucial for the enzyme's function, phosphorylation by casein kinase II enhances cPGES activity 3fold	Rattus norvegicus

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Rattus norvegicus

Protein Variants

Protein Variants	Comment	Organism
A151G	shows similar activity as the wild-type	Rattus norvegicus
S113A	fails to be activated by casein kinase II	Rattus norvegicus
S118A	fails to be activated by casein kinase II	Rattus norvegicus
Y14F	shows similar activity as the wild-type	Rattus norvegicus

Inhibitors

Inhibitors	Comment	Organism	Structure
5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole	-	Rattus norvegicus
dexamethasone	anti-inflammatory glucocorticoid	Rattus norvegicus
geldanamycin	-	Rattus norvegicus
SB-203580	blocks the association of cPGES with casein kinase II and Hsp90, as well as cPGES phosphorylation	Rattus norvegicus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0149	-	prostaglandin H2	cPGES plus casein kinase II plus Hsp90	Rattus norvegicus
0.0357	-	prostaglandin H2	cPGES plus casein kinase II	Rattus norvegicus
0.0666	-	prostaglandin H2	cPGES alone	Rattus norvegicus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	-	Rattus norvegicus	5829	-

Organism

Organism	UniProt	Comment	Textmining
Rattus norvegicus	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
fibroblast	3Y1 cell	Rattus norvegicus	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
2	-	cPGES alone	Rattus norvegicus
2.2	-	cPGES plus casein kinase II	Rattus norvegicus
2.5	-	cPGES plus casein kinase II plus Hsp90	Rattus norvegicus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
Prostaglandin H2	-	Rattus norvegicus	Prostaglandin E2	-	?

Synonyms

Synonyms	Comment	Organism
CPGES	-	Rattus norvegicus
Prostaglandin E synthase	-	Rattus norvegicus

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Release 2023.1 (January 2023)