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Literature summary for 5.3.99.3 extracted from

  • Thoren, S.; Weinander, R.; Saha, S.; Jegerschoeld, C.; Pettersson, P.L.; Samuelsson, B.; Hebert, H.; Hamberg, M.; Morgenstern, R.; Jakobsson, P.J.
    Human microsomal prostaglandin E synthase-1: purification, functional characterization, and projection structure determination (2003), J. Biol. Chem., 278, 22199-22209.
    View publication on PubMed
Search for more literature for 5.3.99.3

Cloned(Commentary)

Cloned (Comment) Organism
expression of histidine-tagged mPGES-1 in Escherichia coli Homo sapiens

General Stability

General Stability Organism
activity in solubilized membranes remains stable for a prolonged time in the presence of reduced glutathione and glycerol Homo sapiens
resistant to freeze-thawing Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.16
-
 (5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate
-
 Homo sapiens
0.16
-
 prostaglandin G2
-
 Homo sapiens
0.71
-
 glutathione
-
 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
 Homo sapiens
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
17500
-
 x * 17500, SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged mPGES-1, hydroxyapatite, Ni2+-affinity chromatography Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.04
-
 substrate 15-hydroperoxy-prostaglandin E2 Homo sapiens
0.043
-
 substrate 5-hydroperoxyeicostetraenoic Homo sapiens
0.17
-
 substrate cumene hydroperoxide Homo sapiens
0.81
-
 substrate 1-chloro-2,4-dinitrobenzene Homo sapiens
120
-
 substrate (5Z,13E)-(15S)-9a,11a-epidioxy-15-hydroxyprosta-5,13-dienoate Homo sapiens
190
-
 substrate prostaglandin G2 Homo sapiens

Storage Stability

Storage Stability Organism
-20°C, 6 months, no loss of activity Homo sapiens
4°C, solubilized membranes, 10 d, 50% loss of activity Homo sapiens
4°C, solubilized membranes, 24 h, 30% loss of activity Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(5Z,13E)-(15S)-9a,11a-epidioxy-15-hydroxyprosta-5,13-dienoate + GSH i.e. prostaglandin H2 Homo sapiens (5Z,13E)-(15S)-11a,15-dihydroxyprosta-5,13-dienoate + GSSG i.e. prostaglandin E2 ?
1-chloro-2,4-dinitrobenzene + GSH glutathione S-transferase activity Homo sapiens ?
-
 ?
15-hydroperoxy-prostaglandin E2 + GSH
-
 Homo sapiens prostaglandin E2 + GSSG
-
 ?
5-hydroperoxyeicosatetraenoic acid + 2 GSH
-
 Homo sapiens 5-hydroxyeicosatetraenoic acid + GSSG + H2O
-
 ?
cumene hydroperoxide + 2 GSH
-
 Homo sapiens cumene hydroxide + GSSG + H2O
-
 ?
prostaglandin G2 + GSH
-
 Homo sapiens 15-hydroperoxy-prostaglandine E2 + GSSG
-
 ?

Subunits

Subunits Comment Organism
? x * 17500, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
mPGES-1
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
21
-
 glutathione
-
 Homo sapiens
50
-
 (5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate
-
 Homo sapiens
75
-
 prostaglandine G2
-
 Homo sapiens