Literature summary for 5.3.99.2 extracted from

Yamamoto, K.; Higashiura, A.; Suzuki, M.; Aritake, K.; Urade, Y.; Nakagawa, A.
Molecular structure of a prostaglandin D synthase requiring glutathione from the brown planthopper, Nilaparvata lugens (2017), Biochem. Biophys. Res. Commun., 492, 166-171 .

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Activating Compound

Activating Compound	Comment	Organism	Structure
glutathione transferase	-	Nilaparvata lugens

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli Rosetta (DE3) pLysS cells	Nilaparvata lugens

Crystallization (Commentary)

Crystallization (Comment)	Organism
in complex with glutathione, sitting drop vapor diffusion method, using 0.1 M Tris-HCl, pH 8.0, 28% (w/v) PEG4000	Nilaparvata lugens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.32	-	GSH	at pH 8.0, temperature not specified in the publication	Nilaparvata lugens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
Prostaglandin H2	Nilaparvata lugens	-	Prostaglandin D2	-	?

Organism

Organism	UniProt	Comment	Textmining
Nilaparvata lugens	J9Q529	strain Izumo	-

Purification (Commentary)

Purification (Comment)	Organism
DEAE-Sepharose column chromatography and Superdex 200 gel filtration	Nilaparvata lugens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1-chloro-2,4-dinitrobenzene + GSH	-	Nilaparvata lugens	?	-	?
additional information	the enzyme is inactive toward 1,2-epoxy-3-(4-nitrophenoxy)-propane, 4-nitrophenethyl bromide, 4-nitrobenzyl chloride, 4-hydroxynonenal, ethacrynic acid, and 4-nitrophenyl acetate	Nilaparvata lugens	?	-	?
Prostaglandin H2	-	Nilaparvata lugens	Prostaglandin D2	-	?

Subunits

Subunits	Comment	Organism
homodimer	2 * 23000, SDS-PAGE	Nilaparvata lugens

Synonyms

Synonyms	Comment	Organism
PGDS	-	Nilaparvata lugens
Prostaglandin D synthase	-	Nilaparvata lugens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	-	Nilaparvata lugens

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
1.7	-	GSH	at pH 8.0, temperature not specified in the publication	Nilaparvata lugens

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Release 2023.1 (January 2023)