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Literature summary for 5.3.99.2 extracted from

  • Fukuhara, A.; Yamada, M.; Fujimori, K.; Miyamoto, Y.; Kusumoto, T.; Nakajima, H.; Inui, T.
    Lipocalin-type prostaglandin D synthase protects against oxidative stress-induced neuronal cell death (2012), Biochem. J., 443, 75-84.
    View publication on PubMed
Search for more literature for 5.3.99.2

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) Homo sapiens

Protein Variants

Protein Variants Comment Organism
C65A/C167A site-directed mutagenesis Homo sapiens
C89A/C167A/C186A site-directed mutagenesis Homo sapiens
C89A/C186A site-directed mutagenesis Homo sapiens
additional information knockdown of the enzyme expression by siRNA in SH-SY5Y cells, two different siRNAs Homo sapiens
Y63S/T67S/C89A/C186A site-directed mutagenesis Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum rough endoplasmic reticulum membrane Homo sapiens 5783
-
extracellular secreted to the cerebrospinal fluid Homo sapiens
-
-
membrane rough endoplasmic reticulum membrane Homo sapiens 16020
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens lipocalin-type prostaglandin D synthase is a dual-functional protein, acting as a prostaglandin D2-producing enzyme and a lipid transporter, the enzyme can bind a wide variety of lipophilic molecules ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens P41222
-
-

Source Tissue

Source Tissue Comment Organism Textmining
arachnoid mater
-
 Homo sapiens
-
brain synthesized mainly at the rough endoplasmic reticulum membrane of arachnoid cells, chorioid plexus cells and oligodendrocytes, and then is secreted into the cerebrospinal fluid as a second major protein Homo sapiens
-
cerebrospinal fluid second major protein Homo sapiens
-
choroid plexus
-
 Homo sapiens
-
neuron
-
 Homo sapiens
-
oligodendrocyte
-
 Homo sapiens
-
SH-SY5Y cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information lipocalin-type prostaglandin D synthase is a dual-functional protein, acting as a prostaglandin D2-producing enzyme and a lipid transporter, the enzyme can bind a wide variety of lipophilic molecules Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
More structure homology modelling using the solution structure of the C65A mouse L-PGDS, PDB code 2RQ0, as template, overview Homo sapiens

Synonyms

Synonyms Comment Organism
L-PGDS
-
 Homo sapiens
lipocalin-type PGD synthase
-
 Homo sapiens
lipocalin-type prostaglandin d synthase
-
 Homo sapiens
Prostaglandin D synthase
-
 Homo sapiens
Prostaglandin-H2 D-isomerase UniProt name Homo sapiens

Expression

Organism Comment Expression
Homo sapiens enzyme expression is increased in H2O2-treated neuronal cells up

General Information

General Information Comment Organism
evolution the enzyme is a member of the lipocalin superfamily Homo sapiens
additional information structure homology modelling using the solution structure of the C65A mouse L-PGDS, PDB code 2RQ0, as template, overview Homo sapiens
physiological function the enzyme shows a protective effect on H2O2-induced apoptosis in neuroblastoma cell line SH-SY5Y, the enzyme level is highly associated with H2O2-induced apoptosis. It protects against neuronal cell death by scavenging reactive oxygen species without losing its ligand-binding function. H2O2 reacts with the thiol of Cys65 of the enzyme Homo sapiens