Cloned (Comment) | Organism |
---|---|
for expression in Escherichia coli cells | Mus musculus |
Crystallization (Comment) | Organism |
---|---|
mutant DELTA1-24_C65A L-PGDS is crystallized in two different crystal forms representing the conformational change between the open and closed states of the cavity of the beta-barrel, the structures are determined to resolutions of 2.1 and 2.0 A | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
DELTA1-24_C65A | mutant | Mus musculus |
DELTA1-24_C65A+H111A | mutant | Mus musculus |
DELTA1-24_C65A+P110A | mutant | Mus musculus |
DELTA1-24_C65A+W54A | mutant | Mus musculus |
DELTA1-24_L-PGDS | mutant | Mus musculus |
DELTA1-24_L-PGDS+H111A | mutant | Mus musculus |
DELTA1-24_L-PGDS+H116A | mutant | Mus musculus |
DELTA1-24_L-PGDS+P110A | mutant | Mus musculus |
DELTA1-24_L-PGDS+S45A | mutant | Mus musculus |
DELTA1-24_L-PGDS+S45A/S81A | mutant | Mus musculus |
DELTA1-24_L-PGDS+S45A/T67A | mutant | Mus musculus |
DELTA1-24_L-PGDS+S45A/T67A/S81A | mutant | Mus musculus |
DELTA1-24_L-PGDS+S81A | mutant | Mus musculus |
DELTA1-24_L-PGDS+T67A | mutant | Mus musculus |
DELTA1-24_L-PGDS+T67A/S81A | mutant | Mus musculus |
DELTA1-24_L-PGDS+W45A | mutant | Mus musculus |
DELTA1-24_L-PGDS+W54A/H111A | mutant | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
U-46619 | - |
Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0005 | - |
prostaglandin H2 | mutant DELTA1-24_L-PGDS+S45A | Mus musculus | |
0.0008 | - |
prostaglandin H2 | mutant DELTA1-24_L-PGDS | Mus musculus | |
0.0008 | - |
prostaglandin H2 | mutant DELTA1-24_L-PGDS+W54A/H111A | Mus musculus | |
0.0012 | - |
prostaglandin H2 | mutant DELTA1-24_L-PGDS+S45A/S81A | Mus musculus | |
0.0012 | - |
prostaglandin H2 | mutant DELTA1-24_L-PGDS+S45A/T67A/S81A | Mus musculus | |
0.0013 | - |
prostaglandin H2 | mutant DELTA1-24_L-PGDS+W45A | Mus musculus | |
0.0014 | - |
prostaglandin H2 | mutant DELTA1-24_L-PGDS+H111A | Mus musculus | |
0.0014 | - |
prostaglandin H2 | mutant DELTA1-24_L-PGDS+H116A | Mus musculus | |
0.0015 | - |
prostaglandin H2 | mutant DELTA1-24_L-PGDS+S81A | Mus musculus | |
0.0016 | - |
prostaglandin H2 | mutant DELTA1-24_L-PGDS+P110A | Mus musculus | |
0.0021 | - |
prostaglandin H2 | mutant DELTA1-24_L-PGDS+S45A/T67A | Mus musculus | |
0.0021 | - |
prostaglandin H2 | mutant DELTA1-24_L-PGDS+T67A/S81A | Mus musculus | |
0.0028 | - |
prostaglandin H2 | mutant DELTA1-24_L-PGDS+T67A | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Prostaglandin H2 | Mus musculus | - |
Prostaglandin D2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | O09114 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Prostaglandin H2 | - |
Mus musculus | Prostaglandin D2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
L-PGDS | - |
Mus musculus |
lipocalin type prostaglandin D synthase | - |
Mus musculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
activity assay | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
activity assay | Mus musculus |
General Information | Comment | Organism |
---|---|---|
physiological function | lipocalin type prostaglandin D synthase is a multi-functional protein acting as a somnogen (PGD2)-producing enzyme, an extracellular transporter of various lipophilic ligands, and an amyloid-beta chaperone in human cerebrospinal fluid | Mus musculus |