Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 5.3.99.2 extracted from

  • Liu, J.; Lin, K.; Guo, C.; Gao, H.; Yao, Y.; Lin, D.
    Expression and purification of cysteine mutation isoforms of rat lipocalin-type prostaglandin D synthase for nuclear magnetic resonance study (2008), Acta Biochim. Biophys. Sin., 40, 489-496.
    View publication on PubMed
Search for more literature for 5.3.99.2

Application

Application Comment Organism
synthesis optimized expression protocol for recombinant enzyme in Escherichia coli and purification protocol yielding large amounts of isotopically labeled enzyme Rattus norvegicus

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
C186A mutant is properly folded with well-defined tertiary structures Rattus norvegicus
C65A mutant is properly folded with well-defined tertiary structures Rattus norvegicus
C65A/C89A/C186A mutant is properly folded with well-defined tertiary structures Rattus norvegicus
C89A/C186A mutant is properly folded with well-defined tertiary structures Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
19000
-
 x * 19000, SDS-PAGE Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
 isoform lipocalin-type prostaglandin D synthase
-

Purification (Commentary)

Purification (Comment) Organism
optimized expression protocol for recombinant enzyme in Escherichia coli and purification protocol yielding large amounts of isotopically labeled enzyme Rattus norvegicus

Storage Stability

Storage Stability Organism
22°C, stable for one month Rattus norvegicus
4°C, stable up to 7 weeks Rattus norvegicus

Subunits

Subunits Comment Organism
? x * 19000, SDS-PAGE Rattus norvegicus