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Literature summary for 5.3.4.1 extracted from

  • Chawsheen, H.A.; Jiang, H.; Ying, Q.; Ding, N.; Thapa, P.; Wei, Q.
    The redox regulator sulfiredoxin forms a complex with thioredoxin domain-containing 5 protein in response to ER stress in lung cancer cells (2019), J. Biol. Chem., 294, 8991-9006 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.3.4.1

Application

Application Comment Organism
diagnostics lung cancer patients with high Srx levels have significantly shorter survival and those with high TXNDC5 levels have longer survival. Cellular levels of Srx and TXNDC5 may be useful as biomarkers to predict the survival of individuals with lung cancer Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
gene TXNDC5, recombinant expression of Myc-tagged wild-type and mutant TXNDC5s in HEK-293T cells, pull-down assay with recombinant, co-expressed FLAG-tagged Srx Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information TXNDC5 knockdown in lung cancer cells generation of TXNDC5 mutants with individual thioredoxin-like domain being deleted. Knockdown of TXNDC5 in lung cancer cells leads to more localization of Srx in the cytosol. Mutation of cysteines in the thioredoxin domains of TXNDC5 leads to the loss of its binding to Srx. Knockdown of TXNDC5 sensitizes human lung cancer cells to endoplasmic reticulum (ER) stress-induced cell death and enhances EGF-induced MAPK activation in in A549 cells Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum
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 Homo sapiens 5783
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Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens TXNDC5 directly interacts with Srx through its thioredoxin-like domains, binding and in vivo complexing analysis. The Srx-TXNDC5 interaction is not affected by the treatment of cells with exogenous H2O2 ?
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Organism

Organism UniProt Comment Textmining
Homo sapiens Q8NBS9
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Source Tissue

Source Tissue Comment Organism Textmining
A-549 cell
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 Homo sapiens
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adenocarcinoma cell
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 Homo sapiens
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bronchioalveolar carcinoma cell
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 Homo sapiens
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cerebral gray matter
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 Homo sapiens
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colon
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 Homo sapiens
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kidney
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 Homo sapiens
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lung cancer cell TXNDC5 is highly expressed in patient-derived lung cancer specimens, immunohistochemic analysis Homo sapiens
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lung squamous cell carcinoma cell
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 Homo sapiens
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additional information TXNDC5 cannot be detected in human normal organs, including bone marrow, prostate, cardiac muscle, and cervix. In contrast, strong positive TXNDC5 staining is consistently observed in tissues from human normal cerebral gray matter, small intestine, colon, stomach, kidney, and salivary gland. No significant numbers of TXNDC5 are positively stained cells in normal lung tissue while strongly positive staining of TXNDC5 is found in lung tumor cells, as evidenced by the dark brown color in examples of lung squamous cell carcinoma, bronchioloalveolar carcinoma, adenocarcinoma, and small cell carcinoma. Differential expression pattern of TXNDC5 in human lung normal and cancer specimens Homo sapiens
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salivary gland
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 Homo sapiens
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small cell lung cancer cell
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 Homo sapiens
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small intestine
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 Homo sapiens
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stomach
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 Homo sapiens
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information TXNDC5 directly interacts with Srx through its thioredoxin-like domains, binding and in vivo complexing analysis. The Srx-TXNDC5 interaction is not affected by the treatment of cells with exogenous H2O2 Homo sapiens ?
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Synonyms

Synonyms Comment Organism
thioredoxin domain-containing protein 5
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 Homo sapiens
TXNDC5
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 Homo sapiens

General Information

General Information Comment Organism
malfunction TXNDC5 knockdown in lung cancer cells inhibits cell proliferation and represses anchorage-independent colony formation and migration, but increases cell invasion and activation of mitogen-activated protein kinases. Knockdown of TXNDC5 sensitizes human lung cancer cells to endoplasmic reticulum (ER) stress-induced cell death. Knockdown of TXNDC5 enhances EGF-induced MAPK activation in human lung cancer cells, which may contribute to the increased invasiveness of these cells Homo sapiens
physiological function the redox regulator sulfiredoxin (Srx) forms a complex with thioredoxin domain-containing 5 protein in response to endoplasmic reticulum (ER) stress, but not to oxidative stress, in lung cancer cells. Increased association of Srx with TXNDC5 facilitates the retention of normally cytosolic Srx in the ER. TXNDC5 directly interacts with Srx through its thioredoxin-like domains Homo sapiens