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Literature summary for 5.3.4.1 extracted from

  • Caba, C.; Khan, H.; Auld, J.; Ushioda, R.; Araki, K.; Nagata, K.; Mutus, B.
    Conserved residues Lys57 and Lys401 of protein disulfide isomerase maintain an active site conformation for optimal activity Implications for post-translational regulation (2018), Front. Mol. Biosci., 5, 18 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.3.4.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
K401Q the mutant shows about 1.5fold less activity than the wild type enzyme Homo sapiens
K57A/K401A the mutant shows about 4fold less activity than the wild type enzyme Homo sapiens
K57E/K401E the mutant shows about 7fold less activity than the wild type enzyme Homo sapiens
K57Q the mutant shows about 1.5fold less activity than the wild type enzyme Homo sapiens
K57Q/K401Q the mutant shows about 2.5fold less activity than the wild type enzyme Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum
-
 Homo sapiens 5783
-

Organism

Organism UniProt Comment Textmining
Homo sapiens P07237
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
acetylation acetylation of the active site-flanking lysine residues can act to reversibly modulate enzyme activity Homo sapiens
S-nitrosylation
-
 Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
Ni-affinity resin column chromatography, and Superdex 200 gel filtration Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dieosin glutathione disulfide
-
 Homo sapiens eosin glutathione sulfide
-
 ?

Synonyms

Synonyms Comment Organism
PDI
-
 Homo sapiens
protein disulfide isomerase
-
 Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Homo sapiens